How to avoid loss of solvent using Dolomite Microfluidics System and maintain stable flow throughout the run?

Hi everyone. I'm currently working with Dolomite Microfluidic system for preparation on PLGA based microparticles, but I seem to be encountering some issues which have been giving me some trouble...

29 June 2020 827 1 View

How to use the optimization tool in matlab (ex : GA, fmincon) to optimize the controller parameter I design in Simulink ?

Hello ! I am doing a research about input shaping control in crane system. The picture below shows that the nonlinear crane system and the input shaping filter I set, and the output are position...

21 November 2019 5,532 5 View

Non-linear effects of varied predator densities?

In my current work, I found a non-linear effect of invasive predators to a native mammal community. That is, the invasive predator had a slightly positive effect to species richness of native...

05 August 2018 2,373 6 View

How to export raw data from WSxM software?

I made a cross section profile of a SEM image via WSxM. However it seems that I can only export it as an image. Does anyone know how to export it into the raw data? Thanks in advance!

07 May 2018 8,617 1 View

The XRD intensity enhanced but FWHM remain the same. Was the FWHM limited by the film thickness?

In my study, I used annealing treatment to improve the crystallinity of thin films. After annealing, the XRD intensity (the (0002) peak in theta two-theta mode) enhanced dramatically but the FWHM...

29 May 2016 8,294 5 View

What cause satellite peaks in XRD measurement?

23 May 2016 747 21 View

Do you have any bioplex beads for bradykinin and eotaxin for measurement in allergic eye disease?

Do you have any bioplex beads for bradykinin and eotaxin for measurement in allergic eye disease? Thank you!

21 January 2016 2,372 1 View

Does anyone know how to obtain mice primary VTA neuron culture?

Protocol needed! Any relative answer will be much appreciated!

03 January 2016 3,415 2 View

How can I make ceramic particles be negative charged in the aqueous solution?

I am studying anodic oxidation of Ti and devoting to coat hydroxyapatite (Ca10(PO4)6OH2) on Ti. During anodic oxidation, Ti was set as anode and a Ti oxide formed on the Ti surface. I would like...

06 September 2015 8,466 3 View

Is TCA extraction method strongly recommended for a Phos-tag gel?

11 February 2015 4,529 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View