We are trying to transfect naive T cells using lentivirus. However the effciency is low (15-20%). Any suggestions to increase the effciency?
My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After several months of storage in a refrigerator...
18 June 2024 7,459 5 View
My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After a month of storage in a refrigerator at 4...
18 June 2024 1,858 1 View
Hello fellow scholars, I would like to know if I measure the drug release of my liposome through a dialysis membrane, how to solve the model that this device may cause [two-stage release]? Of...
09 April 2024 370 0 View
I'm currently working on PSCs. But the conversion efficiency has not improved.
02 January 2024 2,243 0 View
want to know
05 December 2023 4,188 1 View
This is what I got when I searched for SMN1 (survival of motor neuron 1) gene: https://www.ncbi.nlm.nih.gov/gene/6606 However, when I downloaded the sequence data, I found there are 5 gene...
14 August 2023 8,123 0 View
Dear professionals My study is Poly(amide ester) becomes polyimide, I want to compare the change of FTIR spectrum at different temperatures How do I normalize the FTIR spectra? Which peak I can...
07 August 2023 2,437 2 View
I had test the killer effect of the yeast and I found some colonies seem to have an misty inhibition zone rather than a clear inhibition zone on plate as you can see in the picture. Is there any...
29 March 2023 8,954 14 View
Exercise Low Back Pain Related Articles?
24 March 2023 9,507 1 View
I'm doing electrochemical analysis with modified graphite felt as the working electrode and Fe(CN) as the electrolyte. In my CV plot, △Ep became larger, but Ipc/Ipa became close to 1. Is it...
23 December 2022 5,869 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View