Program to find gene copy number in genome?

I am trying to find out how many copies of a gene that I have primers for are in a training set of genomes. I would like program where I enter the genome and the primer (that has degenerate...

15 November 2020 4,523 3 View

Do you have an endnote style for Marine Ornithology?

Any seabird folks out there have an endnote style file for Marine Ornithology? Nothing available online, trying to avoid creating one from scratch - thanks! Perhaps we can make the file publicly...

01 November 2020 9,642 7 View

Storing environmental data with PostgreSQL?

I am wondering if anyone uses postgresql to store data collected by continuous sensors and if there is a pipeline that makes this easier? Also, any other general tips with postgresql would be very...

18 August 2020 939 2 View

Is it necessary to do elution of proteins from Polyacrylamide gel?

Dear researchers, I would like to ask is it necessary to do Elution of proteins from Polyacrylamide gel before sending the protein sample to Nano-LC-TOF-MS Analysis? This is what I plan, I would...

01 August 2020 3,680 3 View

Aside from Mendeley, what other tools/apps can help researchers organize their citations and references?

My question is on looking for suggestions on how citations and references can be easier to generate. Thanks for the response. Hop your sharings could help me.

31 May 2020 5,026 14 View

Hi, i am conducting a legal research on; why people in Ghana are reluctant to commence tort actions like battery in court?

would be very grateful if some recommendations are made on the relevant cases or articles to read to help. thank you.

20 April 2020 4,801 3 View

Regarding on metabolomic?

Dear researchers, May I know how to know what metabolites will be produced by the cells from experiment (Wetlab): 1) Cell (cell culture) 2) Cell infected with virus 3) Cell treated with plant...

31 March 2020 5,865 3 View

How to make elution buffer for SDS-PAGE?

Dear researchers, How to make elution buffer for SDS-PAGE? There is a lot of protocol to make elution buffer, but they didn't show the amount of each ingredient needed. thank you.

25 March 2020 8,333 4 View

Elution of protein from SDS PAGE ?

Dear researchers, May I know do anyone can suggest the protocol of protein elution from SDS PAGE ? Any simple and inexpensive methods? Thank you.

24 March 2020 2,073 13 View

Regarding on Proteomics ?

Dear researchers, I'm currently doing a study regarding on antiviral mechanism of selected plant using metabolomic approach. Plant extract (fruit) will be used and the virus model will be used is...

21 March 2020 5,885 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View