16 Questions 77 Answers 0 Followers
Questions related from Vikrant Mehta
Hello All, Recently we found an interesting observations on cellular signalling events after some drug assays in breast cancer cell lines. The drug seems to be hitting pAkt, however no effect on...
15 March 2024 6,430 0 View
Hi I am culturing SNU398 cells and over the period of time that it displays a two different kind of morphology (one is typical Polygonal kind and the other one is more rounded-like). Do you think...
10 October 2023 8,113 0 View
Hi everyone I just wanted to ask if anyone stored lentivirus particles at -20C. I read that at temperature of -80 they are stable for upto 1-2 years. Does storing them at -20 loose their...
29 July 2022 4,724 4 View
Dear All I am growing MCF10 A cells in the ATCC recommended media (DMEM: F12, 5%Horse serum, etc). I was just wondering if anyone has used the DMEM (or RPMI) with 10% FBS for the same cells? If...
21 December 2021 696 0 View
I am getting a high background in the negative control (Normal secondary IgG) lane. The protocol is the lysate (about 500ug) is mixed with capture antibody and negative antibody overnight at 4C....
07 October 2021 707 0 View
Dear researchers, I am using X-ray films to develop my blots. Even though the bands are good, their quality is getting compromised due to sometimes very fine scratches on X-ray film that may...
26 August 2021 651 0 View
I want to know how to look/analyze different parts in a tumor section like stroma, Ductal epithelial, epithelial lining, luminal epithelial etc etc in a breast cancer tissue slide. Also is there...
03 June 2020 5,300 3 View
Is it necessary that a putative anticancer agent that is altering the mitochondrial membrane potential always leads to alteration in the ROS levels as well? If there is no change in ROS level at...
24 January 2020 4,555 2 View
Hi I found that there are two different recipes for transfer depending on the mode (Semi-dry or wet) of the transfer. Why is it so? If anybody has prior experience with the use of both the...
27 December 2019 1,182 3 View
I performed real-time PCR using an Applied biosystems machine. The double delta Ct values ( according to the machine) for the gene of interest after certain treatments were given as -5.12454;...
24 November 2019 5,542 3 View
The mol wt of my protein of interest is about 27 Kda but i am getting the signal at approx 50Kda. I am Using polyclonal antibody and the standard 12% gel for the detection. Running gel conditions...
03 June 2019 8,798 5 View
I am using a primary antibody (1:500; from cell Signaling Tech) which has been made in BSA. I am using 5% NFDM as blocking solution and then washing the membrane in 1X PBS for 10 minutes and then...
13 January 2019 9,041 2 View
Hi For how much time should cells be incubated with DNA damaging agent(s) like Camptothecin, etoposides so as to get expression of p53 and p21 (At RNA as well as protein level). Any image from a...
03 December 2018 8,695 5 View
Hi I have noticed one thing in cell culture is that the cells love to attach themselves at the side of the the culture vessels more than at any other part of the flask. Also, the these cells...
28 August 2018 1,900 4 View
Hi I am confused regarding the status of HBL 100. Some mention it as 'normal' cell line while others state that it is 'normal like' and another is it is 'transformed'. A clear and precise...
24 August 2018 4,007 3 View
Hi everyone I made a fresh RIPA buffer using foll. composition 1) Tris Buffer 50mM (7.5 pH) 2)150mM NaCl 3)1%NP-40 4)0.5% Sod. deoxycholate 5)Protease inhibitor After lysate was run on SDS-PAGE...
11 June 2018 9,262 5 View
