I think, Western blot analysis of — the amounts of HPV16 E6, E7 proteins to detect the effect of each siRNA in SiHa and CaSki, cervical cancer cells, respectivelyThe following antibodies are recommended to detect HPV16 E6 and E7 proteins for western blot analysis for immunoprecipitation using SiHa and CaSki, cervical cancer cell linescropped gels from western blot analysis of western blot analysis of -use the following antibodies:

For HPV16 E6:

Abcam ab70Mouse monoclonal antibody clone C1P5

ANTI-HUMAN NATIVE HGF CLONE 6F4 MOUSE MONOCLONAL AB805 (Arbor Assays, MI)

For HPV16 E7:

Antibodies: Mouse anti-GAPDH mAb (Invitrogen/Thermo Fisher MA1-16757 & 2322152); Mouse mAb 8C9 (Invitrogen/Thermo Fisher 35-7700)

The mouse antibody clone ED17 (sc-51951) against μ-calpain was purchased from Santa Cruz Biotechnology.

Well Vaidated Antibodies for Detecting HPV16 E6 and E7 Proteins in Western Blot 1. Monoclonal antibodies offer high specificity for the target proteins.

Kazi Ashequr Rahaman Sujan Thank you for your advise!

Choose antibodies that are specific for the HPV16 E6 and E7 proteins to avoid cross-reactivity with other proteins. Some antibodies are identified by a clone number which can indicate their specificity and performance in certain applications. The optimal concentration of the antibody for Western blot may vary, so it's important to follow the manufacturer's recommendations or perform titrations.