I'm implanting Alzet pumps in mice with a brain infusion cannula to deliver into the ICV space. I am familiar with the technique that includes removing the pump from the tubing and then using a syringe to push a dye through the cannula into the brain. The dyes are toxic, and the mice must be euthanized immediately. However, I'd like to visually see the slow infusion coming from the pump, if that is possible. For example, I would put the dye in the pump along with the substance of interest, allow the mouse to survive several hours or overnight, then euthanize and detect the dye. Are there dyes that are non-toxic that would allow this? I know that Evans Blue is used in the blood and India Ink is used in the colon, but I have not been able to verify that they can be used in the brain for a survival surgery.
Hi
I wish you good luck in your search
I offer you the molecule -curcumin- as a natural coloring agent
nontoxic
Dr ZIANI Nouara
Hi mister
You can try with the natural non-toxic coloring which is curcumin
Good luck
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