What kind of programmes/courses does the Health Science/Bioscience dept. run in your institution?

We're a newly set up department (Health Sciences), I'm brainstorming for some potential programmes/courses that we could run. Our team consists of MBBS doctors and PhD holders who have experience...

07 August 2016 2,422 0 View

Does anybody have any experience with using comet assays?

09 October 2014 2,832 6 View

Does anyone have experience with a Leica DM IL microscope- How does one use fluorescence?

07 August 2014 5,892 3 View

Can I preserve my COMET assay slides?

07 August 2014 5,045 18 View

Why is my TMB substrate turning blue before I add it to my wells?

Im doing the ELISA assay, but my TMB substrate is turning pale blue BEFORE i'm adding it to my wells (whilst mixing). Is this a reaction due to exposure to light? How can i avoid this color change?

06 July 2014 5,948 26 View

What does cell culture supernatant mean?

I want to determine TNF-alpha levels in my cell culture. The protocol mentions that i should use Cell Culture Supernatant for this test. There is no mention for the use of any lysis buffer. So...

05 June 2014 6,959 13 View

How do I prepare cell culture lysate for an ELISA?

These questions may sound silly, but this is all quite new to me! I have two queries: 1. If I grow my cells in 24/6-well plates, is it safe to trypsinize the cells first then lyse in buffer? Or...

04 May 2014 1,998 6 View

I need to measure ROS content in my cells using 2' ,7' - dichlorofluorescein diacetate (DCFH-DA). Should this be diluted in DMEM or in DMSO?

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03 April 2014 1,246 6 View

What can I add in my CO2 incubator water reservoir to prevent fungal/bacterial growth?

Usually I add some copper sulphate to my (filtered) autoclaved distilled water in the water reservoir pan, but something orange-white (fungus?) keeps appearing in the water. What else can i add...

03 April 2014 4,133 23 View

Does dead bacteria sink or float in its growth medium?

I'm doing a biofilm assay with streptococcus mitis and mutans. I get excellent biofilm formation after 48-72hr hours incubation (rather than standard 24Hr), but i am worried that this biofilm...

02 March 2014 1,909 8 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View