My cell cultures were exposed for 3 hours to some (very little) sodium azide in the incubator water, will this affect my cells?

04 April 2014 0 4K Report

Do sodium azide fumes react with cells immediately? I quickly replaced the water with fresh autoclaved, but I'm worried that this short exposure (3hrs) may affect my cells. Please advise!

Badges
Science method

More Jhoti Somanah's questions See All

What kind of programmes/courses does the Health Science/Bioscience dept. run in your institution?

We're a newly set up department (Health Sciences), I'm brainstorming for some potential programmes/courses that we could run. Our team consists of MBBS doctors and PhD holders who have experience...

07 August 2016 2,578 0 View

Does anybody have any experience with using comet assays?

Have some basic queries regarding initial setup: 1) Do I need to use the microscope in fluorescence mode? 2) If I put my stained slide on the stage, will the image on the screen remain black, or...

09 October 2014 2,978 6 View

Does anyone have experience with a Leica DM IL microscope- How does one use fluorescence?

We have a Leica DM IL inverted microscope. Can anyone help me out on how to switch to the fluorescence settings? When viewing my stained slides.. Instead of a black background, I am viewing a grey...

07 August 2014 6,048 3 View

Can I preserve my COMET assay slides?

Hi, I'm doing the Alkaline COMET assay. But since I have many samples I wanted to know if it is possible to preserve my slides after doing the electrophoresis? I want to preserve the slides, so...

07 August 2014 5,174 18 View

Why is my TMB substrate turning blue before I add it to my wells?

Im doing the ELISA assay, but my TMB substrate is turning pale blue BEFORE i'm adding it to my wells (whilst mixing). Is this a reaction due to exposure to light? How can i avoid this color change?

06 July 2014 6,093 26 View

What does cell culture supernatant mean?

I want to determine TNF-alpha levels in my cell culture. The protocol mentions that i should use Cell Culture Supernatant for this test. There is no mention for the use of any lysis buffer. So...

05 June 2014 7,096 13 View

How do I prepare cell culture lysate for an ELISA?

These questions may sound silly, but this is all quite new to me! I have two queries: 1. If I grow my cells in 24/6-well plates, is it safe to trypsinize the cells first then lyse in buffer? Or...

04 May 2014 2,128 6 View

I need to measure ROS content in my cells using 2' ,7' - dichlorofluorescein diacetate (DCFH-DA). Should this be diluted in DMEM or in DMSO?

03 April 2014 1,407 6 View

What can I add in my CO2 incubator water reservoir to prevent fungal/bacterial growth?

Usually I add some copper sulphate to my (filtered) autoclaved distilled water in the water reservoir pan, but something orange-white (fungus?) keeps appearing in the water. What else can i add...

03 April 2014 4,678 23 View

What are the best antifungal agents I can use for my cell culture?

I'm currently using amphotericin B, but it seems the fungus is resistant to this. Has anyone used nystatin, clotrimazole, or econazole? If yes, at what dosage? I'm using fibroblast cells.

02 March 2014 7,355 9 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View