Does anybody have any experience with using comet assays?

09 October 2014 2,832 6 View

Does anyone have experience with a Leica DM IL microscope- How does one use fluorescence?

07 August 2014 5,892 3 View

Can I preserve my COMET assay slides?

07 August 2014 5,045 18 View

Why is my TMB substrate turning blue before I add it to my wells?

Im doing the ELISA assay, but my TMB substrate is turning pale blue BEFORE i'm adding it to my wells (whilst mixing). Is this a reaction due to exposure to light? How can i avoid this color change?

06 July 2014 5,948 26 View

What does cell culture supernatant mean?

I want to determine TNF-alpha levels in my cell culture. The protocol mentions that i should use Cell Culture Supernatant for this test. There is no mention for the use of any lysis buffer. So...

05 June 2014 6,959 13 View

How do I prepare cell culture lysate for an ELISA?

These questions may sound silly, but this is all quite new to me! I have two queries: 1. If I grow my cells in 24/6-well plates, is it safe to trypsinize the cells first then lyse in buffer? Or...

04 May 2014 1,998 6 View

I need to measure ROS content in my cells using 2' ,7' - dichlorofluorescein diacetate (DCFH-DA). Should this be diluted in DMEM or in DMSO?

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03 April 2014 1,246 6 View

What can I add in my CO2 incubator water reservoir to prevent fungal/bacterial growth?

Usually I add some copper sulphate to my (filtered) autoclaved distilled water in the water reservoir pan, but something orange-white (fungus?) keeps appearing in the water. What else can i add...

03 April 2014 4,133 23 View

My cell cultures were exposed for 3 hours to some (very little) sodium azide in the incubator water, will this affect my cells?

Do sodium azide fumes react with cells immediately? I quickly replaced the water with fresh autoclaved, but I'm worried that this short exposure (3hrs) may affect my cells. Please advise!

03 April 2014 4,093 0 View

Does dead bacteria sink or float in its growth medium?

I'm doing a biofilm assay with streptococcus mitis and mutans. I get excellent biofilm formation after 48-72hr hours incubation (rather than standard 24Hr), but i am worried that this biofilm...

02 March 2014 1,909 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View