Does anyone have experience isolating microRNA from isolated mitochondria for sequencing?
I changed new primers and water and anealing temperature but i didn't get results?
11 September 2018 3,512 3 View
I was reading through this article. Just felt very bad about the contents which are published by some journals. Looks like there is a lot of garbage, which needs to be identified and...
22 December 2017 3,471 25 View
My aim is to study the micro-structural changes in compacted bentonite for different moisture contents w.r.t time.For that I need to conduct SEM. So, please suggest the precautions I need to...
27 January 2016 5,703 2 View
Any suggestion of nano powders made of natural fibers that can be reinforced with natural fiber polymer matrix composites?
23 November 2015 8,265 8 View
I have isolated and purified a Green algae which was confirmed by its chloroplast 16S rRNA gene sequencing analysis. It is showing 85% sequence similarity with one of the existing algal type...
22 April 2015 5,951 5 View
I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...
22 December 2014 2,981 7 View
I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...
17 July 2014 5,372 2 View
In the era of open access publication and the emergence of a huge number of journals that barely follow publishing ethics (no peer review/pay and publish). It becomes necessary that all journals...
22 February 2014 5,544 6 View
I tried many buffers in achieving my protein in soluble fraction, fortunately I am successful at pH1. Moreover, I have not used any buffer for pH1: its just milliq adjusted to pH1 using conc...
28 October 2013 5,407 1 View
Consider the operation of G is (multiplication).
11 October 2011 1,367 2 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...
21 February 2021 6,633 3 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...
03 February 2021 9,330 3 View
I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...
02 February 2021 8,806 2 View
I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...
27 January 2021 5,145 2 View
Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...
18 January 2021 4,368 3 View
I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...
16 January 2021 3,792 4 View
I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...
09 January 2021 2,320 3 View