Does anyone have experience isolating microRNA from isolated mitochondria for sequencing?
Interested to know if there is any relationship between 1. Tuberculosis and Myocardial Infarction 2. Tuberculosis and COVID-related mortality cases?
11 July 2022 5,018 2 View
ρ∂u/∂t+ρ(u.∇)=∇.[-pI+K]+F I need to add -∂/∂_x (ρU_0 u_j ) this term in all three coordinates instead of F
08 July 2022 480 1 View
Please provide formulas to find parameter fracture strain, Displacement at failure, Stress triaxiality, Strain rate.. Thanks in advance
28 April 2022 112 2 View
I am performing miRNA loss-of-function study in cells and using a reporter assay to validate miRNA inhibition. So, I am planning on cloning the entire 3’-UTR sequence of our miRNA target...
08 August 2021 7,247 3 View
Iam getting these errors when iam analysing AMOVA in Arlequin 1 #[ERROR # 1] from function "Population::New_read_genotypic_data()" : phenotypes have different number of loci 2 #[ERROR # 2] from...
26 April 2021 8,835 3 View
Hi, I am modeling wave propagation in a composite plate using inbuilt PZT-5H. In my model, a signal is actuated at a PZT which is attached to the composite and the wave propagates through the...
16 April 2021 6,071 0 View
I changed new primers and water and anealing temperature but i didn't get results?
11 September 2018 3,639 3 View
I was reading through this article. Just felt very bad about the contents which are published by some journals. Looks like there is a lot of garbage, which needs to be identified and...
22 December 2017 3,585 25 View
What folate supplements are available and the dose that should be administered.
24 May 2016 3,678 16 View
My aim is to study the micro-structural changes in compacted bentonite for different moisture contents w.r.t time.For that I need to conduct SEM. So, please suggest the precautions I need to...
27 January 2016 5,865 2 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...
20 June 2024 9,587 1 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
I am interested in analyzing the effects of some immunotherapy on the number of mitochondria in cancer cells as well as in immune cells. I would like also to isolate mitochondria to use them for...
16 June 2024 9,632 1 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated...
07 June 2024 5,909 0 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...
02 June 2024 5,373 2 View
Hi, are there other methods beside Western Blotting to detect a specific protein? I‘ve isolated mitochondria and want to show that hPar17/Pin4 is present in mitochondria. The Western Blots didn’t...
24 May 2024 5,500 3 View