Ramana Vaka

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Questions related from Ramana Vaka

MicroRNA from isolated mitochondria?

Does anyone have experience isolating microRNA from isolated mitochondria for sequencing?

22 June 2023 3,137 0 View

How do you construct a mutated 3’-UTR miRNA clone to use as a negative control?

I am performing miRNA loss-of-function study in cells and using a reporter assay to validate miRNA inhibition. So, I am planning on cloning the entire 3’-UTR sequence of our miRNA target...

09 August 2021 7,222 3 View