Hiii every one i tried many time but my pcr negative control is contamination?

More Mallela Venkata Ramana's questions See All

What is the main advantage of using observer based fault tolerant controller(FTC) for sensor fault tolerance?

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20 December 2020 1,392 4 View

Is there any software for calculating hit bias (HB), missed precipitation (MP), and false precipitation (FP)?

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27 November 2020 980 2 View

I'm looking for cell culture models for Alzheimer's by targeting amyloid beta peptide, tau protein, bace 1 enzyme ; can anyone give me details please?

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24 November 2020 7,255 5 View

Can someone help me in designing off-MPPT mode in a Standalone DC Microgrid?

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30 May 2020 5,834 1 View

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How to calculate the annealing temperature of a primer pair?

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01 March 2021 360 7 View

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Ashraf Abbas

check the nucleic acid extraction and make sure it is clear and free from PCR inhibitors. In addition to seperate the extraction area from run area

Sibnarayan Datta

in addition to above suggestions, try doing PCR in a different lab (which is not working with the same organism), use their reagents to amplify your samples. Always make the PCR mix in a clean work bench.

best of luck,