Hiii every one i tried many time but my pcr negative control is contamination?

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Ashraf Abbas

check the nucleic acid extraction and make sure it is clear and free from PCR inhibitors. In addition to seperate the extraction area from run area

Sibnarayan Datta

in addition to above suggestions, try doing PCR in a different lab (which is not working with the same organism), use their reagents to amplify your samples. Always make the PCR mix in a clean work bench.

best of luck,

Laura Nguyen

Do you make sure it is a contamination? Whether is it a primer dimer or any byproduct?