Hiii every one i tried many time but my pcr negative control is contamination?

I changed new primers and water and anealing temperature but i didn't get results?

11 September 2018 3,512 3 View

What do you think about such journals, which just publish whatever an author submits?

I was reading through this article. Just felt very bad about the contents which are published by some journals. Looks like there is a lot of garbage, which needs to be identified and...

22 December 2017 3,471 25 View

How can I conduct SEM sample preparation (Compacted Bentonite soil)?

My aim is to study the micro-structural changes in compacted bentonite for different moisture contents w.r.t time.For that I need to conduct SEM. So, please suggest the precautions I need to...

27 January 2016 5,703 2 View

Any suggestion of nano powders made of natural fibers that can be reinforced with natural fiber polymer matrix composites?

Any suggestion of nano powders made of natural fibers that can be reinforced with natural fiber polymer matrix composites?

23 November 2015 8,265 8 View

Could some one help in giving information regarding algal taxonomy?

I have isolated and purified a Green algae which was confirmed by its chloroplast 16S rRNA gene sequencing analysis. It is showing 85% sequence similarity with one of the existing algal type...

22 April 2015 5,951 5 View

Why do I get two bands after digesting my plasmid with PfoI?

I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...

22 December 2014 2,981 7 View

Will my specificity of Restriction Endonuclease BSU36I be affected?

I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...

17 July 2014 5,372 2 View

What is your opinion on grading journals based on quality scientific content?

In the era of open access publication and the emergence of a huge number of journals that barely follow publishing ethics (no peer review/pay and publish). It becomes necessary that all journals...

22 February 2014 5,544 6 View

Can someone advise on the solubility of designed protein?

I tried many buffers in achieving my protein in soluble fraction, fortunately I am successful at pH1. Moreover, I have not used any buffer for pH1: its just milliq adjusted to pH1 using conc...

28 October 2013 5,407 1 View

If f is monomorphism from a group G into itself defined by f(x)=x^3, for all x in G, then can you prove that G is abelian?

Consider the operation of G is (multiplication).

11 October 2011 1,367 2 View

What is the easiest and best way to develop myocardial infarction in laboratory animals? And what materials are used for that?

How is the process of introducing myocardial infarction in laboratory rats and what are the materials required for this and what are the details of that in order to be sure of that?

07 February 2020 6,558 3 View

Looking for the cause of death in myocardial infarcted mice?

10 November 2019 527 3 View

How to import PTB Diagnostic ECG Database in python?

hi my thesis is about myocardial infarction detection. do you know how to import entire database for simulation of research papers?

05 June 2019 6,370 7 View

I want a database for myocardial infarction other than physionet?

hi my thesis is about detection of myocardial infarction from ECG signals and i want to know is there any database for it?

04 May 2019 8,220 9 View

Does stress cause heart attack or stroke?

Research shows how stress can lead to heart attacks and stroke . your valuable comments are highly appreciated. Thanks

03 March 2019 8,389 16 View

Custodiol HTK and Terminal warm blood (Hotshot) any thoughts?

We use Custodiol HTKs for our MIMVS (minimally invasive mitral valve surgery program). In some cases before the x-clamp removal we give 400ml of warm blood but there isn't a SOP for such. In your...

02 March 2019 4,280 7 View

Does stress cause heart attack or stroke?

Research shows how stress can lead to heart attacks and stroke . your valuable comments are highly appreciated. Thanks

02 March 2019 4,042 14 View

How can i get the The PTB Diagnostic ECG Database as a single file?

i want to import it on matlab but i need to download the files individually. how can i download the whole database?

02 March 2019 4,335 4 View

Is there a consensus in the stroke research community on which regions are mainly reliant on the MCA in mice?

Looking for a list of anatomical regions that are very likely to be affected by an occlusion of the MCA in mice.

01 February 2019 6,952 1 View

Macrophages isolated from infarcted mouse myocardium?

Have you isolated macrophages from infarcted myocardial tissue from a mouse? How many macrophages do you expect to get? For example: number of macrophages per gram of infarcted tissue. Any refs or...

31 December 2018 4,468 0 View