Hiii every one i tried many time but my pcr negative control is contamination?

I changed new primers and water and anealing temperature but i didn't get results?

11 September 2018 3,512 3 View

What do you think about such journals, which just publish whatever an author submits?

I was reading through this article. Just felt very bad about the contents which are published by some journals. Looks like there is a lot of garbage, which needs to be identified and...

22 December 2017 3,471 25 View

Any suggestion of nano powders made of natural fibers that can be reinforced with natural fiber polymer matrix composites?

Any suggestion of nano powders made of natural fibers that can be reinforced with natural fiber polymer matrix composites?

23 November 2015 8,265 8 View

Could some one help in giving information regarding algal taxonomy?

I have isolated and purified a Green algae which was confirmed by its chloroplast 16S rRNA gene sequencing analysis. It is showing 85% sequence similarity with one of the existing algal type...

22 April 2015 5,951 5 View

Why do I get two bands after digesting my plasmid with PfoI?

I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...

22 December 2014 2,981 7 View

Will my specificity of Restriction Endonuclease BSU36I be affected?

I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...

17 July 2014 5,372 2 View

What is your opinion on grading journals based on quality scientific content?

In the era of open access publication and the emergence of a huge number of journals that barely follow publishing ethics (no peer review/pay and publish). It becomes necessary that all journals...

22 February 2014 5,544 6 View

Can someone advise on the solubility of designed protein?

I tried many buffers in achieving my protein in soluble fraction, fortunately I am successful at pH1. Moreover, I have not used any buffer for pH1: its just milliq adjusted to pH1 using conc...

28 October 2013 5,407 1 View

If f is monomorphism from a group G into itself defined by f(x)=x^3, for all x in G, then can you prove that G is abelian?

Consider the operation of G is (multiplication).

11 October 2011 1,367 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

No title

01 March 2021 3,905 5 View