How can I conduct SEM sample preparation (Compacted Bentonite soil)?

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MicroRNA from isolated mitochondria?

Does anyone have experience isolating microRNA from isolated mitochondria for sequencing?

21 June 2023 3,146 0 View

Is there any relationship between Tuberculosis and Myocardial Infarction or COVID related Mortality?

Interested to know if there is any relationship between 1. Tuberculosis and Myocardial Infarction 2. Tuberculosis and COVID-related mortality cases?

11 July 2022 5,018 2 View

How to add velocity gradient term in each of the momentum equations ?

ρ∂u/∂t+ρ(u.∇)=∇.[-pI+K]+F I need to add -∂/∂_x (ρU_0 u_j ) this term in all three coordinates instead of F

08 July 2022 480 1 View

How to calculate Fracture strain, Displacement at failure, Stress triaxiality, Strain rate by analytical formula in Ductile damage mode in ABAQUS?

Please provide formulas to find parameter fracture strain, Displacement at failure, Stress triaxiality, Strain rate.. Thanks in advance

28 April 2022 112 2 View

How do you construct a mutated 3’-UTR miRNA clone to use as a negative control?

I am performing miRNA loss-of-function study in cells and using a reporter assay to validate miRNA inhibition. So, I am planning on cloning the entire 3’-UTR sequence of our miRNA target...

08 August 2021 7,247 3 View

Can any one help me out of these errors in Arlequin?

Iam getting these errors when iam analysing AMOVA in Arlequin 1 #[ERROR # 1] from function "Population::New_read_genotypic_data()" : phenotypes have different number of loci 2 #[ERROR # 2] from...

26 April 2021 8,835 3 View

In COMSOL, Can we use "Form an assembly" option while using Piezoelectricity and Solid mechanics?

Hi, I am modeling wave propagation in a composite plate using inbuilt PZT-5H. In my model, a signal is actuated at a PZT which is attached to the composite and the wave propagates through the...

16 April 2021 6,071 0 View

Hiii every one i tried many time but my pcr negative control is contamination?

I changed new primers and water and anealing temperature but i didn't get results?

11 September 2018 3,639 3 View

What do you think about such journals, which just publish whatever an author submits?

I was reading through this article. Just felt very bad about the contents which are published by some journals. Looks like there is a lot of garbage, which needs to be identified and...

22 December 2017 3,585 25 View

Please...,anyone give the guidance to perform SEM and MIP tests on Bentonite clay?

My project is on Analyzing the Ageing effects of expansive clay soil ; Micro-structure approach.For this, I need to perform Scanned Electron Microscopy(SEM) and Mercury Intrusion Porosimetry...

27 November 2015 3,158 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View