Micro-RNA genomic map

More Lena Ilan's questions See All

Protocol for neuraminidase treatment of adherent A549 cells?

Dear all, I am looking for a protocol for neuraminidase (NA) treatment of adherent A549 cells (as a control for SNA and MAL-II staining). I am particularly interested in: - Did you fix the...

22 April 2024 9,938 1 View

How do I prevent dark blue spots/smear and the running front of my BN-PAGE?

Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...

18 April 2024 3,219 0 View

Are cells of the human ovarian cancer cell line HEY positve for the surface marker EpCAM?

Hello everyone, I'm currently establishing a new project using the human ovarian cancer cell line HEY. Unfortunately it turned out that they are negative for both of the two surface markers...

11 September 2023 6,341 2 View

Peppa Pig's episodes transcripts in different languages?

Hi! Does anyone knows if there's a website where I can find Peppa Pig's episodes transcripts in different languages? Or even better, do you know if a parallel corpus including these already...

07 December 2022 1,183 0 View

How to interpret a log transformed dependent variable and a non-transformed independet variable when beta is high?

Dear all! i am not sure how to interpret a log transformed dependent variable Y and a non-transformed independet variable X when beta is high. In my example the regression coefficient (beta) of...

09 November 2022 1,128 3 View

Sample runs out after starting PAGE?

After starting the electrophoresis my samples often 'fall' trough the pocket of the gel and accumulate on the stacking/resolving gel border. Every ingredient of the gel had been prepared fresh,...

07 November 2022 6,489 1 View

Can mycoplasma-infection or treatment with plasmocine affect stem cell differenciation and ion channel functions?

Hello, we had mycoplasma in our stem cell and cardioymyocyte cell culture and after treatment the cells growed very slowly/some were dying (picture) and most differenciations were no longer...

01 March 2022 6,255 2 View

How to stain undifferentiated THP-1s (or other suspension cells) for immunofluorescence microscopy?

Dear everyone, I want to label THP-1 cells for IF microscopy but cannot find any detailed protocol on how to label suspension cells without cytospin - is there at all a way of labelling...

13 December 2021 5,302 3 View

Best way to harvest cells from gelatin-coated plates?

Hello! I'm trying a new protocol, and will be seeding isolated mouse endothelial cells on a 0.1% gelatin-coated 12-well plate. I was wondering if there were any established methods to harvest the...

11 November 2021 9,974 1 View

How to do Western Blot analysis with plasma-derived exosomes?

Hello! Right now I am struggling to perform Western Blot with human, plasma-derived exosomes. I want to confirm the presence of typical, exosomal markers - e.g. Alix, CD63 and CD9. I know that I...

09 November 2021 563 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View