Micro-RNA genomic map

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Is there an programm which includes questionnaires, items and picture presenatation?

Dear all, i have to set up a task for facial emotion recognition which combines questionnaires and a picture presentation. Unfortunately i have no programming skills and could not set a program...

10 November 2020 4,516 3 View

Cell structur in poplar - vessels or rays?

Dear All, I am currently trying to understand the different cell types and structures in poplar wood slices, and I have difficulties distinguishing vessel members from rays in the tangential...

12 October 2020 2,276 2 View

-surf option on gromacs produces weird radial distribution?

Hey guys, the following command uses the -surf flag and it causes some unnormalized-looking g(r) (see attached png file): gmx_mpi rdf -s tpr.tpr -ref 'resname TST' -sel 'resname RER -f movie.xtc...

03 October 2020 4,025 2 View

Surface RDF in Gromacs?

Hello, I am trying to use the -surf option in gmx rdf in order to compute the rdf of certain molecules from the surface (nearest atoms) of my reference molecule . My script is: gmx rdf -s...

22 September 2020 5,097 10 View

COM pulling on gromacs?

Dear all, I am looking for a way to restrain the center of mass (COM) of two different molecules bathed in water, using GROMACS 2018. The only thing close I found on gromacs that does this is the...

07 September 2020 4,435 8 View

Does anyone know what is the best way to transfect HEK cells with many constructs (e.g. 15-20)?

I am trying to transfect HEK cells with 15-20 different plasmids and it seems there is some kind of competition for expression. I am currently using Lipofectamine 2000 and transfect each plasmid...

24 February 2019 6,360 3 View

Best RNA extraction method for full-length cDNA?

I'm trying to generate full-length cDNA of my gene of interest (7kb) but have trouble doing so. I was wondering if maybe there are certain RNA extraction methods that are better suitable for this...

29 January 2019 4,774 4 View

How to prepare a 0.75 M sodium citrate solution with pH 7.0?

I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...

23 October 2018 9,159 6 View

How to make standard curve by using gDNA and plasmid(viral vector) for lenti-virus copy number?

I want to calculate copy number(lentivirus gene in genes of cells) So, i will use two standard curves using WPRE(vector) and Albumin(gDNA) primer. 1. Should plasmid dna be linearized? 2. If so,...

17 June 2018 7,465 3 View

How can I increase protein yield in protein expression?

Hi guys, I'm currently struggling with protein expression... my POI shows very low yield and it seems like the protein expression induction is not very efficient... I already sequenced my...

03 May 2018 9,449 21 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View