Is there an programm which includes questionnaires, items and picture presenatation?

Dear all, i have to set up a task for facial emotion recognition which combines questionnaires and a picture presentation. Unfortunately i have no programming skills and could not set a program...

10 November 2020 4,516 3 View

Cell structur in poplar - vessels or rays?

Dear All, I am currently trying to understand the different cell types and structures in poplar wood slices, and I have difficulties distinguishing vessel members from rays in the tangential...

12 October 2020 2,276 2 View

Does anyone know what is the best way to transfect HEK cells with many constructs (e.g. 15-20)?

I am trying to transfect HEK cells with 15-20 different plasmids and it seems there is some kind of competition for expression. I am currently using Lipofectamine 2000 and transfect each plasmid...

24 February 2019 6,360 3 View

Best RNA extraction method for full-length cDNA?

I'm trying to generate full-length cDNA of my gene of interest (7kb) but have trouble doing so. I was wondering if maybe there are certain RNA extraction methods that are better suitable for this...

29 January 2019 4,774 4 View

How to prepare a 0.75 M sodium citrate solution with pH 7.0?

I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...

23 October 2018 9,159 6 View

How to make standard curve by using gDNA and plasmid(viral vector) for lenti-virus copy number?

I want to calculate copy number(lentivirus gene in genes of cells) So, i will use two standard curves using WPRE(vector) and Albumin(gDNA) primer. 1. Should plasmid dna be linearized? 2. If so,...

17 June 2018 7,465 3 View

How can I increase protein yield in protein expression?

Hi guys, I'm currently struggling with protein expression... my POI shows very low yield and it seems like the protein expression induction is not very efficient... I already sequenced my...

03 May 2018 9,449 21 View

Which filter size should I use for sterile-filtration of aqueous glycerol solutions (10%)?

Hi everyone, I heard that glycerol is better sterile-filtered than autoclaved. Can anybody tell me the appropriate filter size? Thanks in advance!

29 April 2018 5,626 3 View

Studies of wavelets with dimension?

Are there any famous and easy to read, or benchmark study on wavelets, where they use dimensions for time and frequency? If the amplitudes have a correlation to spatial coordinates, for any...

19 May 2017 6,242 1 View

HV Transmission pole (possible scaling and function?)

14 February 2017 7,845 3 View

I'd like to investigate EpCAM and MUC1 expression in MCF7 and MDAMB231 cell lines by flow cytometry. Which brand of antibody do you recommend?

In addition, I'd like to investigate cytokeratin expression in same breast cancer cell lines but I can't decide to use which cytkokeratin?

17 October 2020 5,794 6 View

Does anyone know a rat cell line which expresses EpCAM?

I am looking for a model cell line to validate anti-rat EpCam Antibodies but i am struggling to find a good candidate. Many thanks!

24 August 2020 218 2 View

Aggressive serous epithelial ovarian cancer is potentially propagated by EpCAM+CD45+ phenotype. ?

hello

06 July 2018 7,297 0 View

Can you conjugate antibodies to MACS magnetic beads (MILITENYI) before the incubation with with cells?

I need to shorten my MACS cell isolation protocol as much as I can. When I perform positive selection for EpCAM+ve cells, the militenyi manual says to incubate cells with EpCAM-PE antibody first,...

05 June 2018 6,981 4 View

I am trying to isolate and culture mouse lung epithelial cells. I don't know what to look for under light microscope. Can you share some photos?

I isolate mouse lung epithelial cells. I can see varius morphologies under the light microscope (the purity is ~90% as assesed by flow cytometry for EpCAM+, CD45-ve, CD31-ve population) presumably...

23 May 2018 4,810 1 View

I am trying to isolate and culture mouse lung epithelial cells. I don't know what to look for under light microscope. Can you share some photos?

I isolate mouse lung epithelial cells. I can see varius morphologies under the light microscope (the purity is ~90% as assesed by flow cytometry for EpCAM+, CD45-ve, CD31-ve population) presumably...

04 May 2018 8,349 1 View

Which kind of label free isolation method is best for isolating circulating tumor cells from patient's blood?

08 September 2016 6,894 6 View

Does anybody knows which EpCAM clone is used in the cellsearch platform?

01 February 2016 9,128 4 View

What are specific primers for qPCR measurement for HCC other than AFP?

We've already tried EpCAM, ASGPR, CK-18 and Albumin. They were all positive in healthy blood making them useless. AFP showed great results but we need a second one to increase specifity.

08 September 2015 5,438 3 View

No title

04 May 2015 175 3 View