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Questions related from Lena Ilan
I want to separate polysomic and non-polysomic proteins by sucrose fractionation, and then compare the fractions. If I precipitate the non-polysomic fraction by TCA standards directly from the...
09 October 2013 7,555 6 View
I’m looking for site or software that maps all known micro-RNAs coding sequences. I would like to know if the genomic region that I have got from GWAS coding or any interesting micro-RNA.
10 April 2013 10,028 2 View
My question is about PCR. Simple, nothing special, genotyping PCR. When I’m performing the reaction on my bench, the NTC appears contaminated. On the bench in another lab, with the same pippetors...
27 January 2013 2,073 8 View
I want to knock down CSF-1 (M-CSF) in H358 human lung cancer cells. I bought shRNA from Sigma, a validated one, and checked that it has a seeding sequence in all 4 splicing variants of the CSF...
16 January 2013 9,213 6 View
Hi, I need to calculate a synergy/antagonism between two compounds. One of them, when taken alone, is not toxic, but if you add the non-toxic compound to the toxic one, the mixture causes much...
01 January 1970 4,422 1 View
