Is there an programm which includes questionnaires, items and picture presenatation?

Dear all, i have to set up a task for facial emotion recognition which combines questionnaires and a picture presentation. Unfortunately i have no programming skills and could not set a program...

10 November 2020 4,516 3 View

Cell structur in poplar - vessels or rays?

Dear All, I am currently trying to understand the different cell types and structures in poplar wood slices, and I have difficulties distinguishing vessel members from rays in the tangential...

12 October 2020 2,276 2 View

Does mysticism have a scientific basis?

Mysticism is often treated as the opposite of science. But is it? Please see Natural Laws and Mysticism in the Cosmic Cycle of Creation

25 July 2020 6,696 80 View

Could our fine-tuned universe have been created from a single fine-tuned quantum particle?

Cosmological explanations for our apparently fine-tuned universe are basically divided between a) a vastly huge multiverse of universes with varying fundamental force and mass constants, including...

21 February 2020 4,618 100 View

In SPSS post hoc results of a 2-way ANOVA, are "Multivariate tests" post hoc tests? If so, can the results be corrected by Bonferroni's correction?

Dear all, I am running a RM ANOVA with 2 independent variables (factor A with 3 levels, factor B with 5 levels). The AxB interaction is significant and I want to know using a post hoc test in...

13 March 2019 7,609 5 View

Does anyone know what is the best way to transfect HEK cells with many constructs (e.g. 15-20)?

I am trying to transfect HEK cells with 15-20 different plasmids and it seems there is some kind of competition for expression. I am currently using Lipofectamine 2000 and transfect each plasmid...

24 February 2019 6,360 3 View

Best RNA extraction method for full-length cDNA?

I'm trying to generate full-length cDNA of my gene of interest (7kb) but have trouble doing so. I was wondering if maybe there are certain RNA extraction methods that are better suitable for this...

29 January 2019 4,774 4 View

How to prepare a 0.75 M sodium citrate solution with pH 7.0?

I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...

23 October 2018 9,159 6 View

How to make standard curve by using gDNA and plasmid(viral vector) for lenti-virus copy number?

I want to calculate copy number(lentivirus gene in genes of cells) So, i will use two standard curves using WPRE(vector) and Albumin(gDNA) primer. 1. Should plasmid dna be linearized? 2. If so,...

17 June 2018 7,465 3 View

How can I increase protein yield in protein expression?

Hi guys, I'm currently struggling with protein expression... my POI shows very low yield and it seems like the protein expression induction is not very efficient... I already sequenced my...

03 May 2018 9,449 21 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View