From your experience - what are the biggest impediments in the implementation of Digital Twins?

Even almost 20 years after the first Digital Twin (DT) concept was presented by NASA, there is still no broad implementation of DTs in industry is known to date. Why is that? What is hindering...

29 October 2020 1,003 7 View

Do you know of any publication manuals or style guides that have a chapter on bias-free / inclusive language, aside from APA (7th edition)?

The Publication Manual of APA (7th edition) has a very useful chapter on bias-free language. I would like to know if you've come across such chapters or sections in other publication manuals or...

21 October 2020 1,582 4 View

What can I do to get a straight baseline in RP-HPLC?

I am running a RP-HPLC system with a C18 column. In our group, we mainly use the system to analyze peptides. The baseline of the system is not stable. In every run it is increasing over time. The...

29 July 2020 5,003 11 View

What is the optimal well surface area for C2C12 myoblast differentiation into myotubes?

Does anyone know of studies investigating the optimal well sizes for growth/hypertrophy of C2C12 myoblast-derived myotubes following differentiation using standard serum starvation? I find that...

06 July 2020 598 2 View

Which tube should I use to connect vacuum oil pump with Schlenk line?

I want to connect the vacuum oil pumps in our lab with the Schlenk lines and I am not sure which tubes (material, brand, etc.) I should choose. At the moment I am using a red rubber tube and it is...

30 January 2020 7,354 2 View

Superficiality or shallowness scales for trait evaluation?

Does anyone know if there exist a psychometric scale that measures the personal shallowness/superficiality of an individual when evaluating other's individual traits (age, race, gender,...

11 September 2019 2,566 3 View

How to calculate inter-coder reliability (two coders) with observations fitting almost into the same category?

02 June 2019 979 2 View

How to attach specific bacteria to the surface of micro-spheres/beads?

We are starting up a new project where it would be highly relevant to attach specific bacteria to the surface of micro-spheres/beads of e.g. alginate. However, literature and potential...

18 April 2018 7,993 2 View

A free tool for Deming regression analysis?

Hi, According to this webpage Deming regression can be done in Excel: http://www.real-statistics.com/regression/deming-regression/deming-regression-basic-concepts/ - and seems to work fine. But...

08 November 2017 9,854 2 View

Can I selectively remove host cells from mouse blood while retaining intact bacteria?

To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...

22 October 2017 6,278 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View