Does anyone know of studies investigating the optimal well sizes for growth/hypertrophy of C2C12 myoblast-derived myotubes following differentiation using standard serum starvation? I find that many papers don't report well-sizes for their experiments, however I tend to think that myotube differentiation and growth might be affected by well area, regardless of similar confluence level across different well sizes at the onset of differentiation. Any thoughts/experiences?
I'm not aware of any papers but from personal experience I get C2C12 myoblasts to differentiate well in 6 and 24 well plates. I have not had any luck getting good differentiation in 48 or 96 well plates.
Nice question but not sure if well size affects the process of differentiation. I believe that it should not as cells are grown to more than 80% confluence and then starved or incubated in reduced serum media. Irrespective of size, cells will grow to a certain confluence and then differentiate upon reduction of serum. I have tried different size dishes, flasks and 6, 12 and 24-well plates for both primaries and C2C12 cells, they have differentiated similarly. I
I agree with Patrick Davis but I have never tried smaller well-plates.
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