When looking at microscopy pictures, are there clear definitions when to consider a spore swollen in relation to a resting spore by diameter?
Furthermore, from what point on can a germling considered to be "hyphae"? Can this only be based on branching or maybe also the germtube reaching a certain length?
Transitioning to the consideration of germling differentiation, particularly the identification of hyphae, entails meticulous scrutiny of early developmental stages following spore germination. Conventionally, the characterization of hyphae involves detecting filamentous structures exhibiting characteristics indicative of fungal growth, notably elongation and branching. However, the determination of when a germling attains the status of hyphae may not solely hinge upon branching patterns but may also encompass the attainment of a certain length by the germ tube.
Establishing a definitive boundary for identifying hyphae necessitates contextual considerations encompassing the growth kinetics of the fungal species under scrutiny, as well as environmental variables influencing morphogenetic processes. Consequently, researchers may integrate qualitative observations and quantitative measurements to delineate the transition from germling to hypha, thereby enhancing accuracy and reproducibility in fungal developmental studies.
Hi Jakob, I suggest you read the publications from Utrecht
Article Inter- and intra-species heterogeneity in germination of Asp...
Article The impact of inter- and intra-species spore density on germ...
We develop and manufacture the oCelloScope used to image and analyze fungal spore development over time. What we have chosen when we make algorithms is to have them describe a spore to be germinated once the germtube is visual. There are more good publications and just reach out to ask. Best, Søren
- Badges
- Science method