I am currently working on INS-1E cell line to study pro-inflammatory molecules as well as PDX-1 expression under high glucose. May I know what are the protein isolation protocols for these target proteins? Thank you.
Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...
30 June 2024 1,569 1 View
How to purify boron esters? Hello. I'm synthesizing borates in my lab. My questions are as follows: 1. How to purify boronate esters like B(OR)3 compounds, and it is a UV undetected...
23 May 2024 7,072 2 View
We are currently doing community work where we have to measure the height and weight of children 6-10 years old. The community work is done across different communities. Thus, since the...
03 April 2024 7,311 0 View
Hi All, I would like to seek for your expert input for below queries on RNA QC for RNASeq: Can we proceed for RNASeq run on RNA less than 10ng, with DV200 in between 68 - 80%?Details as below:...
18 March 2024 5,936 2 View
I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...
12 March 2024 9,387 3 View
hi I just start building a nonmem pharmacokinetics model. i got a problem with my estimate result .. is there any reason for %RSE=0?? if anyone know about this issue , please explain this...
04 March 2024 6,077 0 View
【Seeking Insights from Organizational Behavior Scholars】 Dear Esteemed Scholars, I hope this message finds you well. I am currently conducting research in the field of organizational behavior,...
05 February 2024 9,390 6 View
I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...
09 January 2024 7,517 7 View
Looking for somebody who had similar experience when you use DOI issued by ResearchGate. I published one paper in a conference in 2007. The conference seems not to publish their proceeding, and I...
25 December 2023 5,537 1 View
Required a research paper with raw data to conduct a comparison of recent research and future direction
05 December 2023 2,528 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View