Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

Is this typical lymphocyte, the flower cells?

21 September 2020 4,107 3 View

Which is better in determining the effectiveness of artificial licks for wildlife detection in forested areas?

Hello, I'm planning for my future research study on artificial salt lick. The study site that I've selected for this study has been confirmed with no presence of natural salt lick, thus I plan to...

02 August 2020 5,609 1 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

20 July 2020 2,153 3 View

0.5M EDTA buffer preparation: Why does it turn into milky white foam-like mixture?

I am making 200ml of 0.5M EDTA, pH8. I mixed 29.22g EDTA (MW: 292.24g/mol) with 100ml distilled water first (planning to add up to 200ml after measuring pH). I was adding NaOH pellet into the...

03 June 2020 4,581 3 View

How to grow SnO2 nanorods on FTO substrate using hydrothermal method?

i try to immerse SnO2 thin film cotaed-FTO substrate on aqueous solutions (DI water, ethanol and small amount of HCl ), and the experiment is carried out inside lab oven with the temperature of 90...

12 January 2020 4,419 4 View

Which is the best instrument to measure social competence in 12-year-old children?

i am having difficulties in choosing the one and best suitable instrument of social competence for the 12-year-old children. i am planning to combine different questionnaires to measure it, but...

11 December 2019 1,591 3 View

How can I use with ECMWF wind and pressure in MIKE 21 HD modelling and having sinusoidal curve of surface elevation?

I have ECMWF ERA-5 wind and pressure data in NetCDF file, and i was able to convert the data to dsf2 file for use in MIKE 21 HD model. The problem that the results of the simulation (surface...

19 November 2019 3,850 3 View

Apart from western blot, what are the other tests that can be used to determine expression of autophagy-related proteins ?

I would like to know if there are other options that requires less cost.

09 July 2019 6,974 2 View

Social competence scale for grandparents-raising grandchildren?

Hi, I am Hoon. I am currently working on my master thesis on grandparenting and its influences on child's social competence. i failed to find suitable child social competence scale for 12-year-old...

28 March 2019 8,832 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View