Hi All,
I would like to seek for your expert input for below queries on RNA QC for RNASeq:
Can we proceed for RNASeq run on RNA less than 10ng, with DV200 in between 68 - 80%?Details as below:
Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6
ng/uL 4.36 5.36 4.14 4.69 2.85 14.7
A260/280 2.472 2.061 1.636 2.057 1.968 1.968
A260/230 2.044 1.511 0.857 0.837 0.610 2.011
RQS NA NA NA NA NA 2.6
DV200 68.9 80.3 75.5 72.2 80.5 71.8
FYI, the RNA samples were extracted from the laser capture microdissected tissues.
Many thanks in advance!
While these are not the 'ideal' kinds of RNA samples, they should work well enough for sequencing. I have had good success doing RNAseq on LCM samples extracted from FFPE tissue with masses around 1 ng total RNA and DV200% > 25%. Just be sure that your mass quantifications are accurate (i.e., using Qubit).
John Hardy Lockhart Thank you very much for sharing your perspective and advice. This input will guide our decision-making process moving forward. Indeed, we utilize the Qubit Fluorometer 4.0 for RNA quantification purposes.
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