Are there differences between performance of two identical Simple Wes machines?

03 December 2020 8,975 4 View

Does anyone has achieved the installation of gromacs plugin in PyMol, complete in Linux, with its graphic interface?

I am using Ubuntu 20.04 and I have PyMol installed, with a license. But it has been impossible to add the repository ppa:makson96/dynamics. Even downloaded from github, the package with the...

20 September 2020 6,870 1 View

How do I produce a carbon composite consisting of 2 metals?

I have a question in regards to the formation of a carbon composite for wastewater application. I came across some articles that suggest doing co-precipitaition, however those articles requires...

20 September 2020 9,793 5 View

How do I fix my fraction collector, Frac-920 and AktaPurifier?

Hello all, I frequently use the Akta purifier for protein purification and recently have been getting error messages in unicorn saying "error 2012 no response frac, 900". The frac 920 is designed...

02 September 2020 2,757 1 View

Questions pertaining the use of Graphene from sugar: How effective is the coating?

Hi scientific community, I have read some articles pertaining to the use of regular sugar to form graphene composites would like to know some questions pertaining to it Is the composite...

07 August 2020 4,719 3 View

No title

05 August 2020 9,805 2 View

CSTR in series: why is it that 2 CSTR is series performs better then 3?

Hi all, I having some issues with the performance of my reactors. some context to question: 3 CSTR is series All of the reactors are equal in volume Reaction follows first order. (Fenton-based...

13 April 2020 7,459 3 View

Papers on Hop Domestication?

Hello, I am interested in learning about the natural history and domestication of Humulus lupulus. I have been unable to find any papers on this subject, so I am wondering if anybody could...

22 March 2020 4,157 5 View

No color in hot water extract after successive extraction with n-hexane and methanol?

Greetings I'm doing an increasing polarity successive extraction on tree leaves with n-hexane, methanol, and hot water successively. The n-hexane extract gave dark green coloration, while the...

04 March 2020 8,687 3 View

Do you have to clean out the his resin from a column if you are doing successive protein purifications?

30 December 2019 2,906 9 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

Safety of the SARS-Cov-2 mRNA vaccine?

Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...

04 February 2021 721 6 View

Can a mRNA be degraded but still observe a protein over-expression?

Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...

03 February 2021 4,112 4 View