I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA.
What's the purpose of changinf the serum type?
What is in Horse serum and calf serum that promotes the differentiation?
Would just lowering FBS from 10% to 2% and adding 1%NEAA work the same?
Hello Brandon Sousa
To differentiate C2C12 cells, one may have to slow down proliferation of cells and switch to differentiation state. This may be accomplished by supplementing the medium with horse serum instead of FBS because horse serum has a lower content of growth factors and trace elements.
So, using a much lower percentage of horse serum (2%-3%) will ensure that proliferation is slowed down considerably. However, FBS may also be used, but at a lower concentration (0.2% FBS instead of 10%FBS) as it will serve the same purpose.
Best.
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