Are there differences between performance of two identical Simple Wes machines?

03 December 2020 8,975 4 View

How do I produce a carbon composite consisting of 2 metals?

I have a question in regards to the formation of a carbon composite for wastewater application. I came across some articles that suggest doing co-precipitaition, however those articles requires...

20 September 2020 9,793 5 View

How do I fix my fraction collector, Frac-920 and AktaPurifier?

Hello all, I frequently use the Akta purifier for protein purification and recently have been getting error messages in unicorn saying "error 2012 no response frac, 900". The frac 920 is designed...

02 September 2020 2,757 1 View

Questions pertaining the use of Graphene from sugar: How effective is the coating?

Hi scientific community, I have read some articles pertaining to the use of regular sugar to form graphene composites would like to know some questions pertaining to it Is the composite...

07 August 2020 4,719 3 View

CSTR in series: why is it that 2 CSTR is series performs better then 3?

Hi all, I having some issues with the performance of my reactors. some context to question: 3 CSTR is series All of the reactors are equal in volume Reaction follows first order. (Fenton-based...

13 April 2020 7,459 3 View

Papers on Hop Domestication?

Hello, I am interested in learning about the natural history and domestication of Humulus lupulus. I have been unable to find any papers on this subject, so I am wondering if anybody could...

22 March 2020 4,157 5 View

No color in hot water extract after successive extraction with n-hexane and methanol?

Greetings I'm doing an increasing polarity successive extraction on tree leaves with n-hexane, methanol, and hot water successively. The n-hexane extract gave dark green coloration, while the...

04 March 2020 8,687 3 View

Do you have to clean out the his resin from a column if you are doing successive protein purifications?

30 December 2019 2,906 9 View

Disattenuating partial correlations with more than one covariate?

Hi, I'm trying to disattenuate the reliability of a partial correlation. I found a formula (attached) in Multiple Regression in Behavioral Research. However, I'm needing to disattenuate...

25 December 2019 9,250 2 View

No title

Hi i have some question in regards to this kit. My samples contains a blend of micro-algae and bacteria. I would like to to use this kit to quantify the dead/ live cells in my mixed liquor....

14 March 2019 5,691 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Does anyone know what are the pros and cons for using autoencoders instead of CNNs for features extraction in neural networks?

Are there papers that talk about the possible advantages and disadvantages of using autoencoders instead of CNNs for feature extraction in neural network?

15 February 2021 6,952 3 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View