The samples will be collected from the middle of Borneo rainforest so I am trying to find a way to keep the samples from deteriorating for around 2 weeks trip. The method should be simple and light as we will traveling through rainforest.
The best is freezing :)
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...
04 July 2024 4,732 1 View
Hello, I am trying to run Cygwin terminal but I experience a problem of cyproj-13.dll not found as below. I even checked on Cygwin but it is not there. Kindly help.
11 June 2024 4,502 0 View
I've seen many papers on GaAs with a weak density of states at the Fermi level, but more above and below when the conduction band descends and passes through the Fermi level until it crosses and...
09 May 2024 4,503 1 View
I was having a discussion with a friend about the best way to resuspend a cell pellet, flicking the tube or pippeting up and down. I wanted to bring the discussion to this community and ask what...
21 December 2023 4,326 1 View
I have inserted a 300bp sequence of interest into the PTRV2 vector for use in VIGS and transformed that vector into E.coli, plated on LB Agar w/ 1% Kanamycin. There was growth on the plates, but...
31 October 2023 5,559 4 View
I’m not sure why my PCR bands aren’t clean and rectangular. I am using a 1% gel (50 mL TAE buffer with 0.75 g agarose), and I am loading 1 uL of 6x loading dye with 5 uL of PCR sample. I run at...
09 October 2023 3,382 3 View
As OCT creates many downstream issues in the RNA analysis pipeline, has anyone tried thawing OCT tissue (smallish pieces) in prechilled (-20C) RNALATER-ICE? Since OCT is water soluble shouldn't...
24 September 2023 3,436 1 View
I don't understand how I can do it
09 September 2023 3,265 1 View
I have methanol that exceeds the carbonyl content according to ACS specifications. ACS carbonyl test uses formaldehyde, acetone and acetaldehyde as standards, so I have at least one of this...
07 September 2023 7,053 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View