As OCT creates many downstream issues in the RNA analysis pipeline, has anyone tried thawing OCT tissue (smallish pieces) in prechilled (-20C) RNALATER-ICE? Since OCT is water soluble shouldn't the RNAlater remove most of it (provided you have at least 10 volumes) and still relatively maintain RNA integrity (by also increased extraction efficiency?)
We need canals instead of using tubes. The curved canals and stoppers could make changes on waters regime to decrease the speed of the water from topsides to the downsides. In Iranian ancient methods, we used the an ancient canals under the ground that was called Qanat. This means, changing directions with another smaller canals could reduce the speed and the water flows from topside to the downsides
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