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Questions related from Brandon Rosales
I have had some spots in my CHSE-214 cell culture for some time, I do not know if it is a contamination or just cell debris, the medium is not cloudy nor does it show any color change. The cells...
19 March 2025 5,817 2 View
Clone the sequence of a protein in two different vectors, one is pET21a+ which gives a His sequence at the C-terminus and the other vector is pET41A+ which gives His at the C-terminus as well as...
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I need to find a Mammalian cell line that can translate mRNA of a bacterial gene
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I amplified a 2,976 bp sequence so that I could ligate it into a plasmid of approximately 3.6 kb, but when I ligate and then perform the bacterial transformation I only get the plasmid ligation...
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