Does anyone has achieved the installation of gromacs plugin in PyMol, complete in Linux, with its graphic interface?

I am using Ubuntu 20.04 and I have PyMol installed, with a license. But it has been impossible to add the repository ppa:makson96/dynamics. Even downloaded from github, the package with the...

20 September 2020 6,870 1 View

No title

05 August 2020 9,805 2 View

I'm looking for resources on how to use NVivo for analyzing data using Heideggerian hermeneutic phenomenology?

I am currently working on my dissertation and using NVivo to analyze my data. I started line by line hand coding and decided it was going to take too long, so I started using NVivo. I am going...

08 March 2019 2,482 3 View

Optimum conditions for gel electrophoresis of DNA fragment that 3.3kb long?

I want to know what would be best to use for gel electrophoresis of a 3.3kb DNA fragment. I would like to perform a gel extraction and cloning with the fragment. What would be the best voltage...

06 December 2018 3,754 1 View

How can I measure the surface coverage of carbon based nanoparticles on ITO glass?

I am working on the nanopatterning of carbon island deposited on ITO glass substrate and I want to measure the surface area of the glass that is covered by the carbon nanoparticle (number of...

24 September 2017 2,142 3 View

How to optimize solubility of my heterologous protein overexpressed at 0.04mM of IPTG and 26C incubation ?

Good morning/afternoon I am over-expressing a bacterial protein (dimer) in BL21 E. coli, which it works even with 0.04mM of IPTG, incubated at 26C. However, when I want to check the solubility of...

20 August 2017 2,306 5 View

I need the summary of Mattew finkbeiner and his method?

Subliminal priming with nearly perfect performance in the ... Matthew Finkbeiner summary of it and method

17 February 2017 2,388 1 View

What other factors can affect failure of hydrogels to form?

06 April 2015 7,411 4 View

Are you familiar with a freeze thaw method to make hydrogels?

I am currently employing the freeze thaw method to synthesize 10% w/v polvinyl alcohol. However, I cannot get the solution to gel. It becomes a sticky paste instead. I am only using a refrigerator...

20 March 2015 5,015 9 View

Can sebacic acid, lactic acid and acetic anhydride be used as a crosslinker?

I am currently doing a study on poly vinyl alcohol - silica (from rice husk) hydrogels. Unfortunately, I cannot use glutaraldehyde as a crosslinker since it has been banned. In connection to this,...

14 November 2014 4,187 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View