Can someone cite some published research regarding this matter? Well, I need to know the ideal ratio of number of seeds with 10mL bacterial inoculum.
If I am testing the antimicrobial activity of a bacteria in a specific agar (like Nutrient Agar), shouldn't I use the same media for other tests such as biochemical analysis? I am a bit lost as...
03 January 2024 2,956 3 View
Safety of blood donations could be affected due to a donor's previous exposure to transfusion-transmissible infections by a number of various routes. A donor selection criteria must be established...
02 September 2023 3,110 1 View
Greetings, For one of my cognitive sciences lab session, I have to install JupyterLab on my computer (a MacBook Air). However I really am lost with how to do it, especially since I'm not tech...
14 August 2023 4,134 2 View
Clone the sequence of a protein in two different vectors, one is pET21a+ which gives a His sequence at the C-terminus and the other vector is pET41A+ which gives His at the C-terminus as well as...
19 June 2023 9,203 1 View
Will you assist with suggesting peer reviewed articles in support of the initiation of a telehealth platform in a managed care plan to improve the Transition of Care process when a member is...
27 May 2023 6,017 1 View
Hi, I'm trying to create a cylinder by parameters like r_in as it's smaller radius...but it gives me the following error : unable to evaluate expression "r_in". (that's when I gave it...
05 April 2023 5,301 2 View
We are two third year bachelor of psychology students looking into using PsychoPy for a continuous performance task. (CPT) Our project is about the interactions between boredom proneness and...
13 December 2022 3,107 2 View
I need to download and display cloud data specially VFM from CALIOP . could you please help me with this issue? I need the amount of memory I need for that for about only 1-year of data. I already...
05 July 2022 2,015 0 View
Through the usage of PERMA Profiler developed by Butler and Kern (2016) that is model by Dr. Martin Seligman's PERMA Theory of Well Being. Is there a relationship between the well being and...
28 June 2022 2,413 5 View
I want to incubate EVs with cells and then see them in confocal, I think to use covers inside 24-well plates, which minimum volume with EVs can be placed in these wells x 24 h???. And is correct...
04 April 2022 6,471 0 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,300 7 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
Hello, I have a somewhat weird question: has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO? Have you managed to correct it? There is some...
19 July 2024 3,094 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,667 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
10 July 2024 7,143 2 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 406 0 View
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
01 July 2024 8,360 3 View