I want to track large morphological changes over a long period of time by live cell imaging without disturbing the v5-tagged protein structure. so basically I transfect dividing cells with a plasmid harbouring my protein of interest with v5 tag but we cant fuse any fleurescence tag like GFP to protein since it changes its function. I would like to image over time the population that is expressing the v5 and see what is happening to both general structure of cell and localization of protein the same time by live imaging compared to ctrl group.

Any similar experience advanced technical advice about equipment or material is greatly appreciated

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