Yulia Panina

3 Questions 112 Answers 0 Followers

Questions related from Yulia Panina

Can I freeze the SARS-Cov2 spike protein obtained from HEK cells in the form of medium?

I am new to working with viruses, and my task is to infect lung cells with pseudo-virus. The procedure is as follows, 1) Culture HEK293T cells in 35mm dish, transfect with lentiviral spike...

21 May 2022 221 2 View

Toxicity assay not working in ES cells, help?

Dear all, I have human ES cells, I have been exposing them to chemicals like aspirin, amiodarone, chlorpheniramine, chlorpromazine, clotrimazole, 2,4-dinitrotoluene, ibuprofen etc. The cells just...

08 August 2019 7,591 0 View

What problems have you ever encountered while working with multiplex qPCR?

I'm planning to try multiplex qPCR. I found the following description in the past questions: "Beware. Multiplex quantification is very tricky with dozens of pitfalls that will lead to false...

02 May 2019 5,995 3 View