Sepideh Parsi

6 Questions 20 Answers 0 Followers

Questions related from Sepideh Parsi

Can anyone share a protocol please for detecting presence of a hypothetical antibody against a known antigen in mice plasma?

I have hypothesized presence of some antibodies against proteins of interest to likely be present in plasma in disease. How I can quickly check this hypothesis on plasma collected from control and...

28 December 2020 9,385 2 View

IHC H Dab pictures intensity analysis with imageJ issue?

Hi everyone! Now we staying at home so I have all the time for any lengthy protocol even manual. the problem is I add my pictures to the IHC toolbox it just takes ages (nothing appearing after...

17 April 2020 2,128 0 View

Troubleshooting oligodendrocyte APC O4 staining in spinal cord?

Hi, Recently I am trying to stain oligodendrocytes with APC antibody in the mice spinal cord but I only see APC positive cells clearly in young mice below 90days age. Is that normal? I am...

03 January 2020 5,063 0 View

Long live cell imaging friendly anti-V5 tag probe or antibody?

I want to track large morphological changes over a long period of time by live cell imaging without disturbing the v5-tagged protein structure. so basically I transfect dividing cells with a...

22 April 2019 6,203 2 View

How freezing lysate before pulldown assay and after it for studying pr-pr interaction affects the following proteomics analysis?

I have seen in forums different ideas about effect of freezing lysate on following protein analysis methods like MS. I appreciate sharing with me a good reference in this case.

13 December 2017 9,852 2 View

What is the standard protocol for having a cytotoxicity effect caused by Amyloid beta peptides in mouse neural cell culture?

I noticed there are many articles working on this subject, but the method specifically in preparation of Abeta is slightly different from article to article. There were discrepancies about using...

25 September 2014 6,982 4 View