Greetings, recently I have acquired the pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro vector (V012884). I would like to know whether the gene of interest's expression is regulated by the CMV promoter or not, given that this vector contains two sets of 5'LTRs. Specifically, I would like to know which set is integrated into the cell genome subsequent to transduction. Regards in advance,
pCDH-CMV-MCS-EF1-copGFP-T2A-Puro is a dual promoter and dual reporter construct. The CMV promoter drives the expression of the gene of interest cloned into the multiple cloning site (MCS), and the EF1 promoter the expression of the two reporters – GFP and Puro. The self-cleaving T2A peptide sequence is incorporated to mediate the co-expression of GFP and Puro. The GFP reporter indicates expression of the target gene by green fluorescence, while the Puro cassette provides puromycin resistance for selection of stable clones.
