Lentiviral transduction in DAOY, HDMBO3 or ONS-76 cell line?

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The role of higher education to national development?

the role of higher education to national development

13 March 2024 1,954 4 View

Please, what explanation can be proffered for a lower concentration of some drugs being effective in some cancer cell lines?

For example 0.1 μM being more efective than 5 μM. The drugs at different concentrations were added to the cell lines and they were irradiated. The concentrations were not toxic as observed in...

16 March 2023 8,270 2 View

GC COLUMN SELECTION, Can Bpx70 column be used for volatile fatty acids in rumen fluid in aqueous state on GC-FID?

I want to analyze rumen fluid samples in aqueous state for volatile fatty acids on a GC-FID. We only have a Bpx70 column. Can this column be use?

24 September 2020 7,901 4 View

How to prepare Volatile fatty acids standard mixture, from individual acids standard for calibration/quantification of rumen VFA in unknown samples?

I want to prepare Volatile fatty acids standard mixture from individual acids bought from Sigma Aldrich. I have seen different volume mixture contradicting. I need a standard procedure/ volume...

17 September 2020 2,366 3 View

What are the possible impact of work stress on quality care services?

The outcome of care given to the patient may be determined by effective coping ability of health professionals working in critical areas of care.

13 January 2019 9,828 7 View

Step by step laboratory standard procedure for using Standards (eg atropine catechinetc) to determine phytochemicals in plant extract?

i want to know the step by step process and how to eventually construct the graph and calculate the Quantity of the phytochemicals from the curve in mg/g

03 January 2018 9,594 3 View

My lentivirus transduction reporter NC had higher signal than my experimental

Hello, So I got a customized lentivirus (reporter), alongside the postive and negative controls. NB: The negative control contains the reporter sequence without the promoter After transduction, I...

01 January 1970 8,619 1 View

Phronensis online Conference recommendation

Please can any one recommend Phronesis LLC conferences. I am about to register for their ICASVM 2020 conference, so I decide to ask for recommendations

01 January 1970 3,647 1 View

Transfection/ Transduction of IPSc

Hello, I will appreciate if anyone can help with a protocol to either transduce or transfect IPSc as I am planning to do this for the first time Thank you

01 January 1970 3,422 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,850 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View