How to prepare Volatile fatty acids standard mixture, from individual acids standard for calibration/quantification of rumen VFA in unknown samples?

I want to prepare Volatile fatty acids standard mixture from individual acids bought from Sigma Aldrich. I have seen different volume mixture contradicting. I need a standard procedure/ volume...

17 September 2020 2,219 3 View

Are phenols with unsubstituted resorcinol/ phloroglucinol condensed or hydrolysable tannins?

Phytochemical analysis of plant extract, tannin proportion

10 November 2018 6,719 1 View

Can i make use of Potassium ferrocyanide in place of potassium ferricyanide?

Can i make use of Potassium ferrocyanide in place of potassium ferricyanide in the assay of sterol color development for UV-Vis spectrophotometer reading. using it along with sulfuric acid and FeCl3.

03 April 2018 4,210 0 View

Step by step laboratory standard procedure for using Standards (eg atropine catechinetc) to determine phytochemicals in plant extract?

i want to know the step by step process and how to eventually construct the graph and calculate the Quantity of the phytochemicals from the curve in mg/g

03 January 2018 9,471 3 View

Step by step laboratory standard procedure for using Standards (eg atropine catechinetc) to determine phytochemicals in plant extract?

i want to know the step by step process and how to eventually construct the graph and calculate the Quantity of the phytochemicals from the curve in mg/g

31 December 2017 6,822 3 View

How can i freeze my plant extract?

I extracted plant material using 80% ethanol and i want to dry the extract. I have condensed the the extracts on water bath to remove some of the solvent.I put the thick extract in the freezer but...

10 November 2017 8,450 8 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View