Aderonke Olufunke Ajongbolo

4 Questions 6 Answers 0 Followers

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Is this yeast contamination in my IPSC culture?

Hello everyone, I have been working with IPSC for quite a time now and I think I have got a hang of how to culture and work with them. I thawed 2 new vails of cells consecutively and I am seeing...

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Lentiviral transduction in DAOY, HDMBO3 or ONS-76 cell line?

Hello, I want to tranduce the above cell lines using lentivirus. I will appreciate help from anyone that have experience transducing any of these cell lines especially the best MOI and cell number...

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My lentivirus transduction reporter NC had higher signal than my experimental

Hello, So I got a customized lentivirus (reporter), alongside the postive and negative controls. NB: The negative control contains the reporter sequence without the promoter After transduction, I...

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Transfection/ Transduction of IPSc

Hello, I will appreciate if anyone can help with a protocol to either transduce or transfect IPSc as I am planning to do this for the first time Thank you

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