Aderonke Olufunke Ajongbolo

4 Questions 6 Answers 0 Followers

Questions related from Aderonke Olufunke Ajongbolo

Is this yeast contamination in my IPSC culture?

Hello everyone, I have been working with IPSC for quite a time now and I think I have got a hang of how to culture and work with them. I thawed 2 new vails of cells consecutively and I am seeing...

20 February 2025 952 0 View

Lentiviral transduction in DAOY, HDMBO3 or ONS-76 cell line?

Hello, I want to tranduce the above cell lines using lentivirus. I will appreciate help from anyone that have experience transducing any of these cell lines especially the best MOI and cell number...

09 January 2024 778 3 View

My lentivirus transduction reporter NC had higher signal than my experimental

Hello, So I got a customized lentivirus (reporter), alongside the postive and negative controls. NB: The negative control contains the reporter sequence without the promoter After transduction, I...

01 January 1970 8,668 1 View

Transfection/ Transduction of IPSc

Hello, I will appreciate if anyone can help with a protocol to either transduce or transfect IPSc as I am planning to do this for the first time Thank you

01 January 1970 3,475 4 View