Transduction Protocol (using lentivirus or retrovirus)

Materials:

• Lentiviral or retroviral vector
• Polybrene (hexadimethrine bromide)
• iPSCs
• Culture medium (specific to iPSCs)
• Plate (e.g., 6-well or 12-well)

Procedure:

Transfection Protocol (using lipofection)

Materials:

• Lipofection reagent (e.g., Lipofectamine 3000, FuGENE)
• Plasmid DNA or RNA
• iPSCs
• Opti-MEM or other serum-free medium
• Culture medium (specific to iPSCs)
• Plate (e.g., 6-well or 12-well)

Procedure:

Charlie Amoia Thank you for the detailed response, but i have one quick question. Do i dissociate the iPsc into single cells using accutase before plating prior to transduction or transfection?

Thank you

Yes, you typically dissociate induced pluripotent stem cells (iPSCs) into single cells using Accutase before plating them for transduction or transfection. Here's a general procedure you can follow:

Dissociating the cells into a single-cell suspension is crucial for achieving uniform transduction or transfection efficiency.

Charlie Amoia Thank you for the explanation and your time