My lentivirus transduction reporter NC had higher signal than my experimental

Aderonke Olufunke Ajongbolo @Aderonke-Ajongbolo

01 January 1970 1 9K Report

Hello,

So I got a customized lentivirus (reporter), alongside the postive and negative controls. NB: The negative control contains the reporter sequence without the promoter

After transduction, I did the assay and the NC signal was way higher than the experimental signal... I am at a crossroad, I want to know if anyone had experience this and will appreciate any suggestion on how to move forward?

Robert Adolf Brinzer

Is your lentivirus system capable of genomic integration? If so then your negative control could have inserted near a host promoter which is driving it.

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