16 Questions 103 Answers 0 Followers
Questions related from Hamadi Madhi
Hi, I am treating a drug at a different concentration to cancer cells to generate an IC 50 curve. How to make sure 1 ul is added to cell culture with mutichannel pipette?
20 May 2023 4,531 6 View
Hello, Is it necessary to change culture medium before transfection. My cells abruptly lose attachment upon medium change. Thanks.
02 June 2020 4,075 4 View
Hello Is there any optimal condition of centrifugation (speed, time) to obtain cell pellet in a small 1.5mL tube. What I am looking for is a method of harvesting living cells and floating cells...
01 June 2020 5,081 0 View
Hello, I want to study the effect of EGF on the expression of a target protein. Is there any alternative to serum starvation? Do you think that treating cells with a high concentration of EGF in...
26 December 2018 8,981 3 View
Hi,Is there anyone who has a list of the inactivated genes on X-chromosome or at least regions which are silenced. I mean genes which are silenced by XIST in order to achieve dosage...
28 May 2018 7,789 5 View
Hello, Is the permeabilization step essential in Rhodamine 123 mitochondria stainingin order to measure the mitochondrial potential? Do I need to dettach cells with Trypsin before...
12 April 2016 7,176 4 View
Hello, Could you share any reliable protocol for rhodamine 123 staining on adherent cells? Thanks in advance.
02 April 2016 5,647 5 View
Hello dear researchers, I would like to know whether there is someone who has been successful in detecting active-caspase3 and 9 and cleaved PARP using western blot. I would be really grateful...
01 February 2016 9,507 4 View
Hello everyone, I m looking for an Annexin V staining protocol for adherent cells without detaching cells from plate. As known, using trypsin to detach cells can increase considerably the number...
28 July 2015 6,527 2 View
Hello everyone, Hello, I tried recently to transfect the T24 cell line using the Lipofectamine 3000 and the Amaxa nucleofection. Unfortunetly, 24 hours after the transfection 90% of cells were...
16 July 2015 8,968 7 View
Hello everyone, I am working now on optimizing mammospheres culture condition for the MDA-MB231,MCF7 and 4T1 cell lines. My medium is a serum free medium DMEM/F12 supplemented with B27(2X),...
02 June 2015 10,123 2 View
Hello everyone, Anyone has an idea about the pH dissociation method to separate cells? I heard that this method is less harsh on the cells than traditional mechanical(pipetting) or enzymatic...
20 May 2015 7,423 3 View
Is there any alternative to the methylcellulose to abbrogate mammospheres aggregation? Thanks in advance. Hamadi.
04 May 2015 2,333 2 View
Hello everyone, I am trying to grow the 4T1 mammospheres in a 6 well low attachment plate. Does serum have a negative effect on mammosphere growth? Why the mammosphere medium DMEM/HAM F12 must be...
30 April 2015 6,714 4 View
Hi, I would like to know the reason for EGFR internalization upon EGF stimulation in cancer cells. THANK YOU.
01 January 1970 7,042 9 View
Hi, Which growth factor accelerates the division of lung cancer cells? I need an answer specific to lung cancer cells not a general answer. Thanks.
01 January 1970 784 13 View
