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I need a clear and concise explanation
07 June 2024 6,565 3 View
Anyone know of a good replacement for the needle used to puncture gradient tubes on the brandel gradient fractionator? We use this system to fractionate sucrose gradients for polysome profiling,...
07 June 2024 8,212 0 View
I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...
06 June 2024 574 1 View
I have recently run several thermal stepping measurements at a temperature range of +20 to -100 °C, at low light intensity (~500 W/m2) on perovskite solar cells based on MAPbI3 and triple-cation...
05 June 2024 3,761 0 View
I am confused while doing the calculations. My protein concentration is 0.959 ug/uL,1.11 ug/uL and 1.5 ug/uL. I want to load 20 ug/ well and the volume that will go in each well is 30 uL. Total...
05 June 2024 5,206 5 View
I'm dealing with this pond water for analysis of chemical parameters such as (ammonia, nitrite, nitrate, phosphate...etc.) using a visible spectrophotometer while I was preparing standard curves...
04 June 2024 6,727 1 View
I apologize in advance for the length of this post but there is a lot of information. Our lab has an Acquity TQD coupled with an H-Class UPLC (Column manager-CM, sample manager-SM, and quaternary...
04 June 2024 6,326 3 View
Hi everyone, I have some important problems with my western blot against serum proteins. I have used secondary antibody conjugated by HRP that binds in unspecific manner to other proteins as same...
04 June 2024 5,865 8 View
I was wondering if the plasma that is in the top layer after centrifugation can be used to assess proteins, metabolites, hormones, or other molecules, or if the ingredients of the Ficoll alter the...
04 June 2024 1,737 4 View
Am in need of assistance, when #FOUNTAINVALLEYPOLICE #STALK #AND #ARE CHANGING MY GRADE POINT AVERAGE. NO RECORD. AND. POLICE CHOKED ME IN BACK OF POLICE VEHICLE BEFORE ILLEGAL INCARCERATION....
03 June 2024 8,136 1 View
Y=0.0011x+0.1031 (Standard curve eq) Chamomile sample 20mg/mL Absorbance(y)= 1.16 x=960.82(µg/mL) Chamomile sample 10mg/mL Absorbance(y)= 0.80 x=633.55(µg/mL) Tridax sample...
02 June 2024 7,709 0 View
By working on proprioception, we send stimuli to our brain through multiple pathways (senses, muscle spindles, ligaments, etc.) that will help us improve our balance.
02 June 2024 2,215 3 View
Hi everyone Is there any guideline for method validation for cosmetic? I wanna know about criteria for that.
01 June 2024 1,864 2 View
I found the following recipe at Abcam and converted it to 5x to make a stock solution. Running buffer (Tris-Glycine/SDS) 25 mM Tris base 190 mM glycine 0.1% SDS Check the pH and adjust to...
01 June 2024 1,325 2 View
سلام عليكم سؤال هل يشترط عدد الباحثين( ثلاث او اقل ) في اي بحث علمي في مجلة علمية للترقية العلمية اذا كنت الباحث الرابع في البحث العلمي كم نقطة احصل...
01 June 2024 6,003 3 View
Geotechnical engineering
30 May 2024 3,767 1 View
During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.
30 May 2024 5,055 0 View
Is it acceptable to include secondary literature, such as other scoping reviews, umbrella reviews, meta-analyses, or systematic reviews, in the process of conducting a scoping review?
30 May 2024 8,863 2 View
Hello, Does anyone have recommendation on how to minimize signal fluctuation in RapidFire?
30 May 2024 4,978 0 View
Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...
29 May 2024 1,288 0 View
Hi there, I want to get some help, if anyone has already explained and improved the correlation between the crystallite size results and particle size measured by laser granulometry. I have used...
29 May 2024 8,103 3 View
My work is based on fungi growth and for bioactive compound identification and structure purpose, i required LC-MS and GC-MS for my study.
28 May 2024 1,035 1 View
I'm looking for TEM images of osteoclasts that I could use in the online Histology Atlas e-Histology (https://e-histologia.unileon.es/Home/index_en.html) under CC BY license or with permission...
28 May 2024 2,620 0 View
I have successfully run SWAT model in my study area, but I met problems in calibration by SWATCUP. My best result is that R square equals 0.50 and NS equals 0.46, which was low and far from...
28 May 2024 2,263 0 View