For example 0.1 μM being more efective than 5 μM. The drugs at different concentrations were added to the cell lines and they were irradiated. The concentrations were not toxic as observed in Clonogenic assays and CellTitre blue.
Dear Adernoke
It is common that some times, a specific quantity of a drug which is not necessarily the highest concentration, shows more prosperous activity. Various parameters would be involved, however I cannot specify the likely ones in your case with no further information about the nature and mechanism of your drug of interest. As an example, I can refer you to the ethanol 70% which is more effective than 96% one in the sterilization process, because of its more potency of penetration into the microorganisms. Some times solubility or ionic dissociation in lower concentrations is the main issue. In other cases, the change in the function of a drug/substance in different concentration (for inatance shifting from a reductant agent toward an oxidant) should be considered. Good luck
Thank you so much Alireza. I truly appreciate the examples stated. However, one of the drugs acts by inhibiting pyrimidine synthesis and the other inhibits monocarboxylate transporters (MCT1 & MCT4).
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