What is validation of data in HPLC?

In HPLC, what is the validation of data? How to do it?

10 November 2017 9,495 5 View

Can someone provide a recent reference for the estimation of Collagen (Woessner, 1961), DNA (Burton, 1956), Lipid peroxidation (Buege and Aust) etc?

hi, i need recent references for the estimation of Collagen which is similar to the protocol described by Woessner (1961), DNA similar protocol described by Burton, (1956), Lipid peroxidation...

08 September 2017 4,347 3 View

How exactly does bacterial contamination/ the use of antibiotics in media affect cells??

So i have heard that bacteria release toxins to the media and can retard cell growth, but i don't know how exactly it affect the cells and what changes can occur... is it by changing the pH of the...

01 February 2016 2,136 4 View

What is the most important enzyme for human and why?

among the endogenous enzymes like, glutathione, superoxide, catalase and glutatione-s-transferase, what would be the most important for human ?? and what is the reason?? thank you

01 February 2016 4,191 6 View

Is there any way to clean/recover cell lines contaminated with bacteria??

01 February 2016 6,245 4 View

What does it mean when treatment of tumor cell line using plant extract gives biphasic curve?

i have treated a tumor cell line using a plant extract and terminated the treatment at different times (eg: 1 hour, 2 hours, 4 hrs and so on) and estimated some enzyme activities and observed that...

01 February 2016 971 3 View

How to store hela cell homogenates?

i have 102 samples and i have to estimate protein, gsh, lipid peroxidation, gst, gshpx, catalase and sod..... it will take weeks to finish all these assays, so, after making cell homogenates in...

31 December 2015 3,482 2 View

Why plant extracts/drugs have more activity in one cell line as compared to other cell lines?

i have noticed that the in- vitro anticancer activities of my plant extract and an isolated compound differ on different cell lines....... what could be the reason behind?

11 December 2015 3,412 7 View

My MTT reading is fluctuating.. can someone help me?

i have performed MTT as per Mosmann T on HepG2 cell line using different concentrations of plant extracts (1-90 ug/ml). The absorbance of all the treated groups are reduced to about half as...

10 November 2015 9,776 10 View

Hi, is there any facility in india where I can send my plant extracts for in vitro anticancer activity tests?

i need mtt, clonogenic, comet assay etc.

06 July 2015 9,686 7 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View