What is validation of data in HPLC?

In HPLC, what is the validation of data? How to do it?

10 November 2017 9,495 5 View

Can someone provide a recent reference for the estimation of Collagen (Woessner, 1961), DNA (Burton, 1956), Lipid peroxidation (Buege and Aust) etc?

hi, i need recent references for the estimation of Collagen which is similar to the protocol described by Woessner (1961), DNA similar protocol described by Burton, (1956), Lipid peroxidation...

08 September 2017 4,347 3 View

How exactly does bacterial contamination/ the use of antibiotics in media affect cells??

So i have heard that bacteria release toxins to the media and can retard cell growth, but i don't know how exactly it affect the cells and what changes can occur... is it by changing the pH of the...

01 February 2016 2,136 4 View

What is the most important enzyme for human and why?

among the endogenous enzymes like, glutathione, superoxide, catalase and glutatione-s-transferase, what would be the most important for human ?? and what is the reason?? thank you

01 February 2016 4,191 6 View

Is there any way to clean/recover cell lines contaminated with bacteria??

01 February 2016 6,245 4 View

What does it mean when treatment of tumor cell line using plant extract gives biphasic curve?

i have treated a tumor cell line using a plant extract and terminated the treatment at different times (eg: 1 hour, 2 hours, 4 hrs and so on) and estimated some enzyme activities and observed that...

01 February 2016 971 3 View

How to store hela cell homogenates?

i have 102 samples and i have to estimate protein, gsh, lipid peroxidation, gst, gshpx, catalase and sod..... it will take weeks to finish all these assays, so, after making cell homogenates in...

31 December 2015 3,482 2 View

Why plant extracts/drugs have more activity in one cell line as compared to other cell lines?

i have noticed that the in- vitro anticancer activities of my plant extract and an isolated compound differ on different cell lines....... what could be the reason behind?

11 December 2015 3,412 7 View

My MTT reading is fluctuating.. can someone help me?

i have performed MTT as per Mosmann T on HepG2 cell line using different concentrations of plant extracts (1-90 ug/ml). The absorbance of all the treated groups are reduced to about half as...

10 November 2015 9,776 10 View

Can pure DMSO be used as a vehicle in mice?

I need to do anticancer activity and some biochemical parameters of plant extract in mice, my extracts dissolves well in pure DMSO. Is it safe to use pure DMSO as a vehicle if not, what percentage...

09 October 2013 10,027 12 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View