i have treated a tumor cell line using a plant extract and terminated the treatment at different times (eg: 1 hour, 2 hours, 4 hrs and so on) and estimated some enzyme activities and observed that the enzymes decreased up to a certain time, increased again and then decreased again.... how do i interpret this and what exactly is happening??
thank you
You can never be sure of anything when using an "extract" because you do not know what you have in your extracts in terms of compound distribution.
One possibility remains that there are different compounds in your extracts providing you with different responses in your enzymatic assay ...
Best regards
Robert
Hi Lairinzuali,
Well, the funny point is that your plant extract makes the enzyme activity you follow versus time to decrease then to increase, then to decrease again at a longer time of incubation.
A possible explanation is that, as suggested by Robert, a first component in your plant extract "quickly" inhibit the enzyme whom activity you look at. But this component does not act for long (why's that ... its stability??? do you heat the incubation mixture?). Then, another component, acting much less "quickly" begins to act at a longer time and, this time, being more stable, decrease largely yhe atcivity of the enzyme you look at.
A possible further explanation is that the second decrease, that appearing after the inetrmediate re-incrase of the enzyme you follow, is due to a component that is produced during your incubation by some enzyme of the tumor cells transforming a compound of your plant extract to a derivative that is toxic to the enzyme activity you look at. This is OF COURSE very interesting, this hypothesis paths the way to inetresting experments and results.
Best regards
Philippe
thank you Dr.Kiss and Dr.Urban for your valuable answers,,,,, the mixture was incubated at 37 degree celcius, 5% CO2 , and i did not heat beyond this...
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