What is validation of data in HPLC?

In HPLC, what is the validation of data? How to do it?

10 November 2017 9,495 5 View

How exactly does bacterial contamination/ the use of antibiotics in media affect cells??

So i have heard that bacteria release toxins to the media and can retard cell growth, but i don't know how exactly it affect the cells and what changes can occur... is it by changing the pH of the...

01 February 2016 2,136 4 View

What is the most important enzyme for human and why?

among the endogenous enzymes like, glutathione, superoxide, catalase and glutatione-s-transferase, what would be the most important for human ?? and what is the reason?? thank you

01 February 2016 4,191 6 View

Is there any way to clean/recover cell lines contaminated with bacteria??

01 February 2016 6,245 4 View

What does it mean when treatment of tumor cell line using plant extract gives biphasic curve?

i have treated a tumor cell line using a plant extract and terminated the treatment at different times (eg: 1 hour, 2 hours, 4 hrs and so on) and estimated some enzyme activities and observed that...

01 February 2016 971 3 View

How to store hela cell homogenates?

i have 102 samples and i have to estimate protein, gsh, lipid peroxidation, gst, gshpx, catalase and sod..... it will take weeks to finish all these assays, so, after making cell homogenates in...

31 December 2015 3,482 2 View

Why plant extracts/drugs have more activity in one cell line as compared to other cell lines?

i have noticed that the in- vitro anticancer activities of my plant extract and an isolated compound differ on different cell lines....... what could be the reason behind?

11 December 2015 3,412 7 View

My MTT reading is fluctuating.. can someone help me?

i have performed MTT as per Mosmann T on HepG2 cell line using different concentrations of plant extracts (1-90 ug/ml). The absorbance of all the treated groups are reduced to about half as...

10 November 2015 9,776 10 View

Hi, is there any facility in india where I can send my plant extracts for in vitro anticancer activity tests?

i need mtt, clonogenic, comet assay etc.

06 July 2015 9,686 7 View

Can pure DMSO be used as a vehicle in mice?

I need to do anticancer activity and some biochemical parameters of plant extract in mice, my extracts dissolves well in pure DMSO. Is it safe to use pure DMSO as a vehicle if not, what percentage...

09 October 2013 10,027 12 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View