I am getting clot after adding TCA into serum - can anyone help?

I want to estimate lipid peroxidation product i.e. MDA from serum. Method includes addition of TCA in first step but after addition serum is getting cloted. Would you please help me to resolve...

10 November 2013 160 12 View

Can anyone help me calculate the concentration of calcium from serum sample?

I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?

10 November 2013 8,793 2 View

Which biochemical parameters should be measured to assess the status of oxidative stress?

I want to assess the level of oxidative stress in blood. Which are the biochemical marker/markers to be targeted?

08 September 2013 3,322 16 View

How much ul of restriction enzyme is required to digest 10 ul DNA?

Units of my RE -10,000 units. How much RE is required to digest 10ul of DNA?

08 September 2013 6,408 10 View

How can I correlate biochemical parameters with a particular SNP of a gene?

I have determined biochemical parameters like LDL, HbA1C, etc. I want to know how I can correlate these parameters with SNP of the gene?

05 June 2013 4,511 0 View

I am getting two bands after PCR amplification of my gene of interest. What should I do to solve this problem?

I have even tried different annealing temp but I am getting double bands in all the temperatures I have tried.

04 May 2013 8,018 17 View

Can I do cytogenetic studies from blood samples which were stored for 1 year?

I want to study cytogenetics from blood samples. I have never done this type of study so I want to know if it is possible to do cytogenetic study from blood samples which were stored for a year?

03 April 2013 9,609 2 View

Why am I getting smearing after PCR?

I have isolated DNA from bacterial culture.I amplified 16s DNA with PCR. But I am getting smearing after PCR. What could be the problem regarding this? I am attaching photo of my gel.

03 April 2013 3,636 17 View

Help for RT PCR data analysis

I have been trying to analyse my RT PCR data but I am not able to do so, can anyone offer advice?.

02 March 2013 2,343 4 View

Has anyone used RT PCR of Corbett company?

We have Corbett's RT PCR in our lab but I am having difficulty in analysis of data and I am getting amplification in negative control. Can anyone help me ?

02 March 2013 6,440 7 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

No title

02 March 2021 3,060 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View