Is there any report regarding explant culture from marine cone snail?

31 December 2014 2,451 1 View

I am getting clot after adding TCA into serum - can anyone help?

I want to estimate lipid peroxidation product i.e. MDA from serum. Method includes addition of TCA in first step but after addition serum is getting cloted. Would you please help me to resolve...

10 November 2013 160 12 View

Can anyone help me calculate the concentration of calcium from serum sample?

I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?

10 November 2013 8,793 2 View

Which biochemical parameters should be measured to assess the status of oxidative stress?

I want to assess the level of oxidative stress in blood. Which are the biochemical marker/markers to be targeted?

08 September 2013 3,322 16 View

How much ul of restriction enzyme is required to digest 10 ul DNA?

Units of my RE -10,000 units. How much RE is required to digest 10ul of DNA?

08 September 2013 6,408 10 View

How can I correlate biochemical parameters with a particular SNP of a gene?

I have determined biochemical parameters like LDL, HbA1C, etc. I want to know how I can correlate these parameters with SNP of the gene?

05 June 2013 4,511 0 View

I am getting two bands after PCR amplification of my gene of interest. What should I do to solve this problem?

I have even tried different annealing temp but I am getting double bands in all the temperatures I have tried.

04 May 2013 8,018 17 View

Why am I getting smearing after PCR?

I have isolated DNA from bacterial culture.I amplified 16s DNA with PCR. But I am getting smearing after PCR. What could be the problem regarding this? I am attaching photo of my gel.

03 April 2013 3,636 17 View

Help for RT PCR data analysis

I have been trying to analyse my RT PCR data but I am not able to do so, can anyone offer advice?.

02 March 2013 2,343 4 View

Has anyone used RT PCR of Corbett company?

We have Corbett's RT PCR in our lab but I am having difficulty in analysis of data and I am getting amplification in negative control. Can anyone help me ?

02 March 2013 6,440 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View