Is there any report regarding explant culture from marine cone snail?

31 December 2014 2,451 1 View

I am getting clot after adding TCA into serum - can anyone help?

I want to estimate lipid peroxidation product i.e. MDA from serum. Method includes addition of TCA in first step but after addition serum is getting cloted. Would you please help me to resolve...

10 November 2013 160 12 View

Can anyone help me calculate the concentration of calcium from serum sample?

I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?

10 November 2013 8,793 2 View

Which biochemical parameters should be measured to assess the status of oxidative stress?

I want to assess the level of oxidative stress in blood. Which are the biochemical marker/markers to be targeted?

08 September 2013 3,322 16 View

How much ul of restriction enzyme is required to digest 10 ul DNA?

Units of my RE -10,000 units. How much RE is required to digest 10ul of DNA?

08 September 2013 6,408 10 View

How can I correlate biochemical parameters with a particular SNP of a gene?

I have determined biochemical parameters like LDL, HbA1C, etc. I want to know how I can correlate these parameters with SNP of the gene?

05 June 2013 4,511 0 View

I am getting two bands after PCR amplification of my gene of interest. What should I do to solve this problem?

I have even tried different annealing temp but I am getting double bands in all the temperatures I have tried.

04 May 2013 8,018 17 View

Can I do cytogenetic studies from blood samples which were stored for 1 year?

I want to study cytogenetics from blood samples. I have never done this type of study so I want to know if it is possible to do cytogenetic study from blood samples which were stored for a year?

03 April 2013 9,609 2 View

Why am I getting smearing after PCR?

I have isolated DNA from bacterial culture.I amplified 16s DNA with PCR. But I am getting smearing after PCR. What could be the problem regarding this? I am attaching photo of my gel.

03 April 2013 3,636 17 View

Has anyone used RT PCR of Corbett company?

We have Corbett's RT PCR in our lab but I am having difficulty in analysis of data and I am getting amplification in negative control. Can anyone help me ?

02 March 2013 6,440 7 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View