Brijesh Dabhi

17 Questions 38 Answers 0 Followers

Questions related from Brijesh Dabhi

Is there any report regarding explant culture from marine cone snail?

Animal cell culture technique plays a vital role in the establishment of explant culture for production of useful drug compounds related to human diseases.In this prospective, Marine Cone snail...

01 January 2015 2,526 1 View

I am getting clot after adding TCA into serum - can anyone help?

I want to estimate lipid peroxidation product i.e. MDA from serum. Method includes addition of TCA in first step but after addition serum is getting cloted. Would you please help me to resolve...

11 November 2013 232 12 View

Can anyone help me calculate the concentration of calcium from serum sample?

I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?

11 November 2013 8,869 2 View

How much ul of restriction enzyme is required to digest 10 ul DNA?

Units of my RE -10,000 units. How much RE is required to digest 10ul of DNA?

09 September 2013 6,493 10 View

Which biochemical parameters should be measured to assess the status of oxidative stress?

I want to assess the level of oxidative stress in blood. Which are the biochemical marker/markers to be targeted?

09 September 2013 3,418 16 View

How can I correlate biochemical parameters with a particular SNP of a gene?

I have determined biochemical parameters like LDL, HbA1C, etc. I want to know how I can correlate these parameters with SNP of the gene?

06 June 2013 4,565 0 View

I am getting two bands after PCR amplification of my gene of interest. What should I do to solve this problem?

I have even tried different annealing temp but I am getting double bands in all the temperatures I have tried.

05 May 2013 8,078 17 View

Can I do cytogenetic studies from blood samples which were stored for 1 year?

I want to study cytogenetics from blood samples. I have never done this type of study so I want to know if it is possible to do cytogenetic study from blood samples which were stored for a year?

04 April 2013 9,670 2 View

Why am I getting smearing after PCR?

I have isolated DNA from bacterial culture.I amplified 16s DNA with PCR. But I am getting smearing after PCR. What could be the problem regarding this? I am attaching photo of my gel.

04 April 2013 3,763 17 View

How can I correlate results of SNP for my gene of interest with its expression level?

I am studying polymorphism (SNP) of a group of genes in diabetic complications. I got my result of SNP by RFLP. I got my expression analysis result by RT PCR. I want to know how can I correlate...

03 March 2013 5,314 32 View

Has anyone used RT PCR of Corbett company?

We have Corbett's RT PCR in our lab but I am having difficulty in analysis of data and I am getting amplification in negative control. Can anyone help me ?

03 March 2013 6,501 7 View

Help for RT PCR data analysis

I have been trying to analyse my RT PCR data but I am not able to do so, can anyone offer advice?.

03 March 2013 2,399 4 View

What is the purpose of melting curve analysis in RT PCR?

I want to study the expression pattern of my genes of interest. Since i am operating RT PCR for the first time i am not aware of some of the things related to RT PCR. I want to know the...

02 February 2013 426 10 View

How to calculate the Ct value after an RT-PCR run?

I want to study expression analysis of cytokines, for which I have run an RT-PCR. I am using a corbett rotor gene 6000 system. But the thing is that I don't know how to analyze the data obtained...

02 February 2013 3,228 4 View

How can I get a clear sharp band of 20bp of DNA through agarose gel electophoresis?

. Is there any special type of agarose available to get the band of less than 50bp of DNA?

11 November 2012 8,798 6 View

How can I determine the genotype of samples?

I want to determine SNP of control and diabetic patients. I have designed primers for three genes and amplified their DNA with PCR. How can I determine the genotype of these samples after that?

11 November 2012 4,520 24 View

What are the best methods to study gene expression from human blood samples?

I want to study cytokine gene expression from human blood. What is the best method to study gene expression of cytokine?

07 July 2012 1,209 1 View