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Questions related from Brijesh Dabhi
Animal cell culture technique plays a vital role in the establishment of explant culture for production of useful drug compounds related to human diseases.In this prospective, Marine Cone snail...
01 January 2015 2,542 1 View
I want to estimate lipid peroxidation product i.e. MDA from serum. Method includes addition of TCA in first step but after addition serum is getting cloted. Would you please help me to resolve...
11 November 2013 248 12 View
I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?
11 November 2013 8,884 2 View
Units of my RE -10,000 units. How much RE is required to digest 10ul of DNA?
09 September 2013 6,511 10 View
I want to assess the level of oxidative stress in blood. Which are the biochemical marker/markers to be targeted?
09 September 2013 3,434 16 View
I have determined biochemical parameters like LDL, HbA1C, etc. I want to know how I can correlate these parameters with SNP of the gene?
06 June 2013 4,579 0 View
I have even tried different annealing temp but I am getting double bands in all the temperatures I have tried.
05 May 2013 8,091 17 View
I want to study cytogenetics from blood samples. I have never done this type of study so I want to know if it is possible to do cytogenetic study from blood samples which were stored for a year?
04 April 2013 9,686 2 View
I have isolated DNA from bacterial culture.I amplified 16s DNA with PCR. But I am getting smearing after PCR. What could be the problem regarding this? I am attaching photo of my gel.
04 April 2013 3,845 17 View
I am studying polymorphism (SNP) of a group of genes in diabetic complications. I got my result of SNP by RFLP. I got my expression analysis result by RT PCR. I want to know how can I correlate...
03 March 2013 5,321 32 View
We have Corbett's RT PCR in our lab but I am having difficulty in analysis of data and I am getting amplification in negative control. Can anyone help me ?
03 March 2013 6,514 7 View
I have been trying to analyse my RT PCR data but I am not able to do so, can anyone offer advice?.
03 March 2013 2,416 4 View
I want to study the expression pattern of my genes of interest. Since i am operating RT PCR for the first time i am not aware of some of the things related to RT PCR. I want to know the...
02 February 2013 442 10 View
I want to study expression analysis of cytokines, for which I have run an RT-PCR. I am using a corbett rotor gene 6000 system. But the thing is that I don't know how to analyze the data obtained...
02 February 2013 3,242 4 View
. Is there any special type of agarose available to get the band of less than 50bp of DNA?
11 November 2012 8,809 6 View
I want to determine SNP of control and diabetic patients. I have designed primers for three genes and amplified their DNA with PCR. How can I determine the genotype of these samples after that?
11 November 2012 4,529 24 View
I want to study cytokine gene expression from human blood. What is the best method to study gene expression of cytokine?
07 July 2012 1,217 1 View
