I have determined biochemical parameters like LDL, HbA1C, etc. I want to know how I can correlate these parameters with SNP of the gene?
Animal cell culture technique plays a vital role in the establishment of explant culture for production of useful drug compounds related to human diseases.In this prospective, Marine Cone snail...
31 December 2014 2,575 1 View
I want to estimate lipid peroxidation product i.e. MDA from serum. Method includes addition of TCA in first step but after addition serum is getting cloted. Would you please help me to resolve...
10 November 2013 293 12 View
I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?
10 November 2013 8,934 2 View
I want to assess the level of oxidative stress in blood. Which are the biochemical marker/markers to be targeted?
08 September 2013 3,477 16 View
Units of my RE -10,000 units. How much RE is required to digest 10ul of DNA?
08 September 2013 6,577 10 View
I have even tried different annealing temp but I am getting double bands in all the temperatures I have tried.
04 May 2013 8,132 17 View
I want to study cytogenetics from blood samples. I have never done this type of study so I want to know if it is possible to do cytogenetic study from blood samples which were stored for a year?
03 April 2013 9,734 2 View
I have isolated DNA from bacterial culture.I amplified 16s DNA with PCR. But I am getting smearing after PCR. What could be the problem regarding this? I am attaching photo of my gel.
03 April 2013 3,898 17 View
I have been trying to analyse my RT PCR data but I am not able to do so, can anyone offer advice?.
02 March 2013 2,468 4 View
I am studying polymorphism (SNP) of a group of genes in diabetic complications. I got my result of SNP by RFLP. I got my expression analysis result by RT PCR. I want to know how can I correlate...
02 March 2013 5,342 32 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
11 July 2024 1,801 2 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.
23 June 2024 2,287 7 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore
04 June 2024 5,688 2 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
31 May 2024 6,444 1 View
What is the difference between absorption and adsorption?
30 May 2024 4,447 27 View