Is there any qualitative or quantitative method to measure E3 ligase Ubiquitination invitro following drug treatment?

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AnthonyKi Lui

Can you just do an in vitro ubiquitination assay and then separate your protein by SDS gel and then do a western blot to measure the intensity of the smearing using anti-Ub antibody?

Fuad Bahram

Check one of our publication which is describing that but with SKP2

http://www.ncbi.nlm.nih.gov/pubmed/12769844

and also Markus Welcker 2004 he has been working with FBW7