Parash Parajuli

5 Questions 6 Answers 0 Followers

Questions related from Parash Parajuli

Is there any qualitative or quantitative method to measure E3 ligase Ubiquitination invitro following drug treatment?

I would like to know if we can measure E3 ligase FBw7 activity or protein expression in breast cancer cells following drug treatment.

05 May 2013 2,235 2 View

Which cell lysis buffer recipe is best for phosphorylated proteins ?

I would be grateful to receive recipe for extracting total protein from breast cancer cell lines to see expression of phosphorylated proteins.

03 March 2013 1,443 12 View

Why are mice exposed to radiation before inoculation of AML blast in xenograft engraftment?

I was reading a paper on Leukemia Stem cell and could not understand why mice were exposed to radiation before inoculation of CB lin- cells and surrogate neoplastic stem cells.

03 March 2013 6,367 0 View

What would be an easy experiment to check drug action on c-myc activity?

Specifically in breast cancer human (MCF-7 , MDA MB 231) and mouse mammary epithelial cells.

02 February 2013 777 3 View

Which is the best software to design cDNA primers and where is it available?

Tutorials for designing primers and trouble shooters are equally appreciated.

02 February 2013 2,252 4 View